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Recombinant Human Cystatin SA Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1201-PI

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1201-PI-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Inhibition Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Cystatin SA protein
Trp21-Ala141, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp21

Predicted Molecular Mass

16 kDa

SDS-PAGE

16 kDa, reducing conditions

Activity

Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008).
The IC50 value is<8.0 nM, as measured under the described conditions.

Formulation, Preparation and Storage

1201-PI
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 8.0.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin SA

Cystatin SA is a member of family 2 of the cystatin superfamily (1). Together with cystatins S and SN, it is produced by the salivary gland and secreted largely in the submandibular/sublingual saliva (2). Cystatin SA inhibits cathepsin L, but may not inhibit cathepsins B and H (3). The functions of Cystatin SA are largely unknown. However, it has been suggested to play a role in the control of periodontal tissue destruction (3).

References

  1. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  2. Baron, A.C. et al. (1999) Exp. Oral. Biol. 5:344.
  3. Baron, A. et al. (1999) Oral Dis. 5:234.

Alternate Names

CST2, CSTSA

Entrez Gene IDs

1470 (Human)

Gene Symbol

CST2

UniProt

Additional Cystatin SA Products

Product Documents for Recombinant Human Cystatin SA Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Cystatin SA Protein, CF

For research use only

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Reconstitution Calculator

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