Recombinant Human Cystatin B Protein, CF

Name: Recombinant Human Cystatin B Protein, CF
Brand: R&D Systems
SKU: 1408-PI

Catalog # 1408-PI | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

Product Datasheet / COA / SDS

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Key Product Details

Source:	E. coli
Accession #:	P04080
Applications:	Inhibition Activity

View Full Technical Details for Recombinant Human Cystatin B Protein, CF

Technical Data
Citations (4) / Reviews

Product Specifications for Recombinant Human Cystatin B Protein, CF

Source	E. coli-derived human Cystatin B protein Met2-Phe98, with an N-terminal Met and 7-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	12 kDa
SDS-PAGE	12 kDa
Activity	Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (Catalog # ES009). The IC₅₀ value is <15 nM, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1408-PI

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile 25 mM Tris and 100 mM NaCl, pH 7.5.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cystatin B

Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Mutations in the Cystatin B gene is the cause of progressive myoclonus epilepsy (EPM1) (3). Because of its expression patterns, Cystatin B can be used as a marker for certain cancers, such as glioblastoma tumors (4). It readily forms amyloid fibrils in vitro (5). The human Cystatin B consists of 98 amino acid residues (3).

Item	Value
References	Abrahamson, M. (1994) Methods Enzymol. 244:685. Pol, E. and I. Bjork (1999) Biochemistry 38:10519. Pennacchio, L.A. et al. (1996) Science 271:1731. Zhang, R. et al. (2003) Glia 42:194. Zerovnik, E. et al. (2002) Biochim. Biophys. Acta 1594:1.
Entrez Gene IDs	1476 (Human); 13014 (Mouse)
Alternate Names	CPI-B, CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B, CSTB, Cystatin B, EPM1, Liver thiol proteinase inhibitor, PME, Stefin B, cystatin B (stefin B), stefin-B

Citations for Recombinant Human Cystatin B Protein, CF (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Cystatin B Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Hu et al. (2019-05-17), Proximity ligation assays for precise quantification of femtomolar proteins in single cells using self-priming microfluidic dPCR chip Anal. Chim. Acta, 2019-01076(0):118-124.
PMID: 31203955
Species: Human

Kasabova M et al. (2014-04-30), Regulation of TGF-beta1-driven differentiation of human lung fibroblasts: emerging roles of cathepsin B and cystatin C. J Biol Chem, 2014-0289(23):16239-51.
PMID: 24790080
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay

Cheng T et al. (2006-03-24), Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification. J. Biol. Chem., 2006-0281(23):15893-9.
PMID: 16565075
Sample Types: Protein
Applications: Enzyme Assay

Johnson GD et al. (2005-10-21), Characterization of cathepsin L secreted by Sf21 insect cells. Arch. Biochem. Biophys., 2005-1444(1):7-14.
PMID: 16274658

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