Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

Name: Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF
Brand: R&D Systems
SKU: 342-CD
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 342-CD

Citations (7)

(1)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	P10747
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

Complete Your Research

Product Specifications for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

Source

Mouse myeloma cell line, NS0-derived CD28 protein

Human/Cynomolgus Monkey/Rhesus Macaque CD28 (Asn19-Pro152) Accession # P10747	DMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Asn19

Predicted Molecular Mass

42.2 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human/Cynomolgus Monkey/Rhesus Macaque CD28 Fc Chimera is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1) that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

342-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD28

CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant co-stimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig
V-like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is up-regulated rapidly following T cell activation and CD28 ligation. Cell surface expression of mouse CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the
up-regulation of bcl-X_L. CD28 ligation has also been shown to regulate Th1/Th2 differentiation.

Item	Value
References	Lenschow, D.J. et al. (1996) Annu. Rev. Immunol. 14:233. Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131. Ward, S.G. (1996) Biochem. J. 318:361.
Entrez Gene IDs	940 (Human); 12487 (Mouse); 102146670 (Cynomolgus Monkey)
Alternate Names	CD28, CD28 antigen, CD28 antigen (Tp44), CD28 molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44

Citations for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF (7)

Showing 1 - 7 of 7 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Hanson et al. (2020), ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models PLoS ONE, 15(9):e0239595.
PMID: 32970735
Species: N/A
Sample Types: Protein
Applications: Bioassay

UA Ramagopal et al. (2017), Structural basis for cancer immunotherapy by the first-in-class checkpoint inhibitor ipilimumab Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 28484017
Sample Types: Protein
Applications: Bioassay

Haanstra K et al. (2015), Selective blockade of CD28-mediated T cell costimulation protects rhesus monkeys against acute fatal experimental autoimmune encephalomyelitis. J Immunol, 194(4):1454-66.
PMID: 25589073
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kowalczyk A et al. (2014), Cell-extrinsic CTLA4-mediated regulation of dendritic cell maturation depends on STAT3. Eur J Immunol, 44(4):1143-55.
PMID: 24338929
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Sakthivel P et al. (2007), The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm. J. Intern. Med., 261(4):399-407.
PMID: 17391115
Species: Human
Sample Types: Serum
Applications: ELISA (Capture)

Neuber K et al. (2006), Autoantibodies against CD28 are associated with atopic diseases. Clin. Exp. Immunol., 146(2):262-9.
PMID: 17034578
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Lazetic S et al. (2002), Chimeric co-stimulatory molecules that selectively act through CD28 or CTLA-4 on human T cells. J. Biol. Chem., 277(41):38660-8.
PMID: 12167647

There are no citations that match your criteria.

Showing 1 - 7 of 7 citations Showing All

Customer Reviews for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF (1)

4 out of 5

1 customer rating

5 stars

4 stars

100%

3 stars

2 stars

1 star

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

Name: Melissa Kemp

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 09/12/2020

Protein:protein interaction by Octet. Immobilize CD28-Fc and monitor interaction of CD80. Fitting KD ~2-3uM

There are no reviews that match your criteria.

Showing 1 - 1 of 1 review Showing All

Product Documents for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: CD28

Citations for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF (7)

Customer Reviews for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF (1)

Customer Images

Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

Product Documents for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected