Recombinant Human CRELD1 Protein, CF

Name: Recombinant Human CRELD1 Protein, CF
Brand: R&D Systems
SKU: 3128-CR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3128-CR

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Key Product Details

Source:	NS0
Accession #:	Q96HD1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human CRELD1 Protein, CF

Product Specifications for Recombinant Human CRELD1 Protein, CF

Source	Mouse myeloma cell line, NS0-derived human CRELD1 protein Gln30-Glu362, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained: Gln30 predicted, sequencing might be blocked
Predicted Molecular Mass	36.8 kDa
SDS-PAGE	50-55 kDa, reducing conditions
Activity	Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.3-1.2 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3128-CR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CRELD1

Cysteine-rich with EGF-like domain protein 1 (CRELD1) is an approximately 50 kDa transmembrane glycoprotein that contains a 333 amino acid (aa) extracellular domain (ECD), two tandem transmembrane segments, and a second ECD of 15 aa (1). Alternative splicing of human CRELD1 generates an additional isoform with a substitution that encompasses the transmembrane segments and the C‑terminal ECD (1). CRELD1 is widely expressed during development and in the adult (1). It interacts with Reticulon 3/RTN3 and inhibits the pro‑apoptotic activity of RTN3 (2). CRELD1 also associates with Adrenergic alpha ₁ receptors in the prostate and limits their expression (3). Missense mutations of human CRELD1 are associated with susceptibility to atrioventricular septal defects in the heart (4). R&D Systems in‑house testing indicates that CRELD1 mediates the adhesion of ATDC-5 cells.

Item	Value
References	Rupp, P.A. et al. (2002) Gene 293:47. Xiang, R. and S. Zhao (2009) Mol. Cell. Biochem. 331:225. Nishimune, A. et al. (2010) J. Pharmacol. Sci. 113:169. Robinson, S.W. et al. (2003) Am. J. Hum. Genet. 72:1047.
Long Name	Cysteine-rich Protein With EGF-like Domains 1
Entrez Gene IDs	78987 (Human); 171508 (Mouse); 312638 (Rat)
Alternate Names	AVSD2, CIRRIN, CRELD1, DKFZp566D213, atrioventricular septal defect 2, cysteine-rich with EGF-like domain protein 1, cysteine-rich with EGF-like domains 1

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Recombinant Human CRELD1 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human CRELD1 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: CRELD1

Citations for Recombinant Human CRELD1 Protein, CF

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Product Datasheets

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