Recombinant Human CRELD1 Protein, CF

Catalog # 3128-CR | R&D Systems, Inc. a Bio-Techne Brand
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3128-CR-050
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Key Product Details

Accession #

Q96HD1

Source

NS0

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CRELD1 protein
Gln30-Glu362, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln30 predicted, sequencing might be blocked

Predicted Molecular Mass

36.8 kDa

SDS-PAGE

50-55 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 0.3-1.2 μg/mL.

Formulation, Preparation and Storage

3128-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRELD1

Cysteine-rich with EGF-like domain protein 1 (CRELD1) is an approximately 50 kDa transmembrane glycoprotein that contains a 333 amino acid (aa) extracellular domain (ECD), two tandem transmembrane segments, and a second ECD of 15 aa (1). Alternative splicing of human CRELD1 generates an additional isoform with a substitution that encompasses the transmembrane segments and the C‑terminal ECD (1). CRELD1 is widely expressed during development and in the adult (1). It interacts with Reticulon 3/RTN3 and inhibits the pro‑apoptotic activity of RTN3 (2). CRELD1 also associates with Adrenergic alpha1 receptors in the prostate and limits their expression (3). Missense mutations of human CRELD1 are associated with susceptibility to atrioventricular septal defects in the heart (4). R&D Systems in‑house testing indicates that CRELD1 mediates the adhesion of ATDC-5 cells.

References

  1. Rupp, P.A. et al. (2002) Gene 293:47.
  2. Xiang, R. and S. Zhao (2009) Mol. Cell. Biochem. 331:225.
  3. Nishimune, A. et al. (2010) J. Pharmacol. Sci. 113:169.
  4. Robinson, S.W. et al. (2003) Am. J. Hum. Genet. 72:1047.

Long Name

Cysteine-rich Protein With EGF-like Domains 1

Alternate Names

AVSD2, CIRRIN

Entrez Gene IDs

78987 (Human); 171508 (Mouse); 312638 (Rat)

Gene Symbol

CRELD1

UniProt

Q96HD1

Product Documents for Recombinant Human CRELD1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human CRELD1 Protein, CF

For research use only

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