Product Specifications for Recombinant Human Contactin-4 Protein, CF
Mouse myeloma cell line, NS0-derived human Contactin-4 protein Asp19-Gly1001, with a C-terminal 10-His tag
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
145-155 kDa, reducing conditions
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 400 ng on a nitrocellulose-coated microplate.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Contactin-4 (CNTN4), also known as BIG-2 (Brain-derived Immunoglobulin Superfamily molecule 2), is an axonal cell adhesion molecule that belongs to the contactin family, a subfamily of the Ig superfamily (1 - 3). The contactin family comprises six members (CNTN1/F3, CNTN2/TAG-1, CNTN3/BIG-1, CNTN4/BIG-2, CNTN5/NB-2 and CNTN6/NB-3) and are characterized by the presence of six Ig-like domains, four fibronectin type III-like repeats, and a glycosylphosphatidylinositol (GPI)-anchoring domain. Contactin family proteins exist as membrane-bound proteins, but can also be released as soluble proteins by GPI-specific phospholipase D. Soluble Contactins are functionsl proteins that are able to promote neurite outgrowth. Human CNTN4 has been mapped to chromosome 3p26 - p25. Three alternative transcripts of CNTN4, encoding isoforms a, b, and c precursor proteins containing 1026, 282, and 698 amino acid (aa) residues, respectively, have been described. Human CNTN4 isoform a shares 94% aa sequence identity with its rat homolog. It also shares from 44 - 66% aa sequence identity with other CNTN family members. CNTN family members display overlapping but distinct expression patterns. CNTN4 expression is detected in multiple organs including brains, pancreas, kidney, aorta, small intestine, thyroid, uterus and testis. However, expression of the 282 aa isoform b is primarily restricted to the brain. CNTN4 has been suggested to play important roles in the formation of neuronal networks during nervous sytems development. Disruption of CNTN4 has been implicated in the 3p deletion syndrome characterized by growth failure, developmental delay, and mental retardation. CNTN-4 expression is induced in human neuroblastoma tumor cells treated with retinoic acid and may be responsible for the the neuritogenic response of tumor cells to retinoids. R&D Systems' recombinant CNTN4 shows in vitro neurite outgrowth promoting activity when used as an immobilized substrate for hippocampal neuron outgrowth.
Fernandez, T. et al. (2004) Am. J. Genet. 74:1286.
Hasnford, L.M. et al. (2003) Cytogenet. Genome Res. 101:17.
Yoshihara, Y. et al. (1995) J. Neurobiology 28:51.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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