Recombinant Human Complement Factor H-related 4/CFHR4, CF New

Name: Recombinant Human Complement Factor H-related 4/CFHR4, CF
Brand: R&D Systems
SKU: 11300-CH

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 11300-CH

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HEK293, His-tag

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Key Product Details

Source:	HEK293
Accession #:	Q92496.3
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Complement Factor H-related 4/CFHR4, CF

Product Specifications for Recombinant Human Complement Factor H-related 4/CFHR4, CF

Source	Human embryonic kidney cell, HEK293-derived human Complement Factor H-related 4/CFHR4 protein Glu20-Glu578, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Glu20
Predicted Molecular Mass	64 kDa
SDS-PAGE	92-103 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Mouse Complement Component C3d His-tag (Catalog # 2655-C3) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human Complement Factor H-related 4/CFHR4 His-tag (Catalog # 11300-CH) binds with an ED₅₀ of 1.50-15.0 ng/mL.

Scientific Data Examples for Recombinant Human Complement Factor H-related 4/CFHR4, CF

	Recombinant Human Complement Factor H‑related 4/CFHR4 His-tag Protein Binding Activity. When Recombinant Mouse Complement Component C3d His-tag (2655-C3) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human Complement Factor H‑related 4/CFHR4 His-tag Protein (Catalog # 11300-CH) binds with an ED₅₀ of 1.50-15.0 ng/mL.
	Recombinant Human Complement Factor H-related 4/CFHR4 His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human Complement Factor H-related 4/CFHR4 His-tag Protein (Catalog # 11300-CH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 92-103 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11300-CH

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Factor H-related 4/CFHR4

Complement factor H-related protein 4 (CFHR4) is a secreted glycoprotein member of the factor H family of glycoproteins (1). The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (FHR-1 to -5) (1). The genes of this family are located on human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (2). CFHRs are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. All CFHRs, including CFHR4, are capable of binding complement factor C3b, discriminate between self and non-self cell surfaces, and have been proposed to deregulate complement activation by inhibiting interaction of CFH with C3b and C3d (1, 3). CFHR4 activates complement through its interaction with C3b (4). CFHR4 can also bind to native (pentameric) C-reactive protein (pCRP) through its first SCR domain and is suggested to promote pCRP binding to necrotic cells surfaces, thus promoting cell clearance (5). CFHR4 is synthesized as 2 isoforms by hepatocytes and circulates in plasma. The shorter isoform, FHR4B, contains 5 of 9 SCRs. The CFHR4 product includes a duplication insertion of 247 aa and represents the longer alternative splice form termed FHR4A (6).

Item	Value
References	Skerka, C. et al. (2013) Mol. Immunol. 56:170. Diaz-Guillen, M.A. et al. (1999) Immunogenetics 49:549. Hellwage, J. et al. (1999) FEBS Lett. 462:345. Hebecker, M. and J. Jozsi (2011) J. Biol. Chem. 287:19528. Mihlan, M. et al. (2009) Mol. Immunol. 46:335. Jozsi, M. et al. (2005) Eur. J. Hum. Genet. 13:321.
Entrez Gene IDs	10877 (Human)
Alternate Names	complement factor H-related protein 4, CFHL4, CFHR4, Complement Factor H-related 4, Complement Factor Hrelated 4, FHR-4, FHR4

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Recombinant Human Complement Factor H-related 4/CFHR4, CF New

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human Complement Factor H-related 4/CFHR4, CF

Scientific Data Examples for Recombinant Human Complement Factor H-related 4/CFHR4, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Complement Factor H-related 4/CFHR4

Citations for Recombinant Human Complement Factor H-related 4/CFHR4, CF

Product Documents for Recombinant Human Complement Factor H-related 4/CFHR4, CF

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