Recombinant Human Complement Factor D Protein, CF

Name: Recombinant Human Complement Factor D Protein, CF
Brand: R&D Systems
SKU: 1824-SE
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1824-SE

Citations (2)

(1)

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Key Product Details

Source:	NS0
Accession #:	P00746
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human Complement Factor D Protein, CF

Product Specifications for Recombinant Human Complement Factor D Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Complement Factor D/Adipsin protein Ile26-Ala253, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ile26 & His35
Predicted Molecular Mass	26 kDa & 25 kDa
SDS-PAGE	27 kDa & 26 kDa, reducing conditions
Activity	Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >70 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1824-SE

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl₂ and Glycerol.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Factor D/Adipsin

Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin (1). It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3 (2). Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity (3).

Item	Value
References	White, R.T. et al. (1992) J. Biol. Chem. 267:9210. Taylor, F.R. et al. (1999) Biochemistry 38:2849. Kim, S. et al. (1995) J. Biol. Chem. 270:24399.
Entrez Gene IDs	1675 (Human); 11537 (Mouse); 54249 (Rat)
Alternate Names	ADN, ADNcomplement factor D, AMBP-1, Adipsin, C3 convertase activator, CFD, Complement Factor D, D component of complement (adipsin), DF, EC 3.4.21, EC 3.4.21.46, PFD, Properdin factor DADIPSIN, complement factor D (adipsin), complement factor D preproprotein

Citations for Recombinant Human Complement Factor D Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Complement Factor D Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Aye I et al. (2014-01-01), Adiponectin inhibits insulin function in primary trophoblasts by PPARalpha-mediated ceramide synthesis. Mol Endocrinol, 28(4):512-24.
PMID: 24606127
Schipper HS et al. (2010-01-01), A multiplex immunoassay for human adipokine profiling. Clin. Chem., 56(0):1320.
PMID: 20530731

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Customer Reviews for Recombinant Human Complement Factor D Protein, CF (1)

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Recombinant Human Complement Factor D Protein, CF

Name: Jenna Nguyen

Application: Apoptosis assay

Verified Customer | Posted 07/27/2018

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Product Documents for Recombinant Human Complement Factor D Protein, CF

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Recombinant Human Complement Factor D Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human Complement Factor D Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Complement Factor D/Adipsin

Citations for Recombinant Human Complement Factor D Protein, CF (2)

Customer Reviews for Recombinant Human Complement Factor D Protein, CF (1)

Recombinant Human Complement Factor D Protein, CF

Product Documents for Recombinant Human Complement Factor D Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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