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Recombinant Human Coagulation Factor Xa Protein, CF

Catalog # 1063-SE | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Size / Price

Key Product Details


Sf 21 (baculovirus)

Accession #

Structure / Form

Active form




Enzyme Activity

Product Specifications


Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Coagulation Factor X protein
Leu24-Lys488 with a C-terminal 10-His tag
Proform Factor X was expressed, purified, activated and further purified to yield Factor Xa.


>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr84, Phe124 & Ile235

Predicted Molecular Mass

12 kDa & 30 kDa


13-14 kDa and 33-36 kDa, reducing conditions


Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >700 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl and CaCl2.
Reconstitution Reconstitute at 100 μg/mL in sterile 25 mM MES, 150 mM NaCl and 5 mM CaCl2, pH 6.0.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor X

As the only known physiological activator of thrombin, Factor X is a vitamin K-dependent plasma protease that plays a pivotal role in blood coagulation. Human Factor X is initially synthesized in the liver as a single-chain precursor of 488 amino acid residues with a signal peptide and a pro region (residues 1‑40). Both the intrinsic and extrinsic pathways activate Factor X to Xa, which consists of light (residues 41‑179) and heavy (residues 235‑488) chains linked by a disulfide bond. The light chain contains a Gla and two EGF-like domains and the heavy chain corresponds to the serine protease domain. The full-length human Factor X was expressed and the pro enzyme was purified and activated. The active protease (rhXa) was further purified and analyzed for its activity towards either peptides or proteins containing a Xa cleavage site. In addition to the activity described in the Activity Assay Protocol, rhFX also be used to cleave fusion proteins containing a Factor Xa cleavage site. The conditions for the optimal cleavage of a particular fusion protein, such as the molar ratio between rhFX and the protein and time and temperature of incubation, are protein-dependent and need to be individually determined.

Alternate Names

Cf10, F10, FX, FXa, Prothrombinase, Stuart Factor

Entrez Gene IDs

2159 (Human); 14058 (Mouse); 29243 (Rat)

Gene Symbol



Additional Coagulation Factor X Products

Product Documents for Recombinant Human Coagulation Factor Xa Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Coagulation Factor Xa Protein, CF

For research use only

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    Application: Enzymatic activity in vitro
    Verified Customer | Posted 08/03/2016

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