Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Recombinant Human Coagulation Factor Xa Protein, CF

Catalog # 1063-SE | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
1063-SE-010

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Structure / Form

Active form

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Coagulation Factor X protein
Leu24-Lys488 with a C-terminal 10-His tag
Proform Factor X was expressed, purified, activated and further purified to yield Factor Xa.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr84, Phe124 & Ile235

Predicted Molecular Mass

12 kDa & 30 kDa

SDS-PAGE

13-14 kDa and 33-36 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >700 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

1063-SE
Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl and CaCl2.
Reconstitution Reconstitute at 100 μg/mL in sterile 25 mM MES, 150 mM NaCl and 5 mM CaCl2, pH 6.0.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor X

As the only known physiological activator of thrombin, Factor X is a vitamin K-dependent plasma protease that plays a pivotal role in blood coagulation. Human Factor X is initially synthesized in the liver as a single-chain precursor of 488 amino acid residues with a signal peptide and a pro region (residues 1‑40). Both the intrinsic and extrinsic pathways activate Factor X to Xa, which consists of light (residues 41‑179) and heavy (residues 235‑488) chains linked by a disulfide bond. The light chain contains a Gla and two EGF-like domains and the heavy chain corresponds to the serine protease domain. The full-length human Factor X was expressed and the pro enzyme was purified and activated. The active protease (rhXa) was further purified and analyzed for its activity towards either peptides or proteins containing a Xa cleavage site. In addition to the activity described in the Activity Assay Protocol, rhFX also be used to cleave fusion proteins containing a Factor Xa cleavage site. The conditions for the optimal cleavage of a particular fusion protein, such as the molar ratio between rhFX and the protein and time and temperature of incubation, are protein-dependent and need to be individually determined.

Alternate Names

Cf10, F10, FX, FXa, Prothrombinase, Stuart Factor

Entrez Gene IDs

2159 (Human); 14058 (Mouse); 29243 (Rat)

Gene Symbol

F10

UniProt

Additional Coagulation Factor X Products

Product Documents for Recombinant Human Coagulation Factor Xa Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Coagulation Factor Xa Protein, CF

For research use only

Citations for Recombinant Human Coagulation Factor Xa Protein, CF (2)

Showing  1 - 2 of 2 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Coagulation Factor Xa Protein, CF include:

There are no citations that match your criteria.

Showing  1 - 2 of 2 publications Showing All

Customer Reviews for Recombinant Human Coagulation Factor Xa Protein, CF (1)

5 out of 5
1 customer rating
5 stars
100%
4 stars
0%
3 stars
0%
2 stars
0%
1 star
0%
Showing  1 - 1 of 1 review Showing All
Filter By:
  • Recombinant Human Coagulation Factor Xa Protein, CF
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 08/03/2016

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Loading...
Setting the standard in quality research reagents