Recombinant Human CMG-2 Protein, CF

Name: Recombinant Human CMG-2 Protein, CF
Brand: R&D Systems
SKU: 2940-CM
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 2940-CM

Citations (1)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	P58335
Applications:	Binding Activity

View Full Technical Details for Recombinant Human CMG-2 Protein, CF

Product Specifications for Recombinant Human CMG-2 Protein, CF

Source	Mouse myeloma cell line, NS0-derived human CMG-2/ANTXR2 protein Gln34-Asn317, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained: Gln34 predicted
Predicted Molecular Mass	31.4 kDa
SDS-PAGE	35-40 kDa, reducing conditions
Activity	Measured by its ability to bind anthrax protective antigen (PA) in a functional ELISA. Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2940-CM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CMG-2/ANTXR2

Capillary Morphogenesis Gene-2 (CMG-2) is a widely expressed anthrax toxin receptor (ATR) family protein (1 - 3). CMG-2 is a 55 kDa type I transmembrane (TM) protein that contains a 33 amino acid (aa) signal sequence, a 284 aa extracellular domain (ECD), a 24 aa TM segment, and a 147 aa cytoplasmic domain. There are three additional isoforms. Isoforms 4 shows a 12 aa insertion in the cytoplasmic region; isoform 2 shows a 103 aa deletion in the ECD; and isoform 3 is a truncated, 20 kDa, 289 aa soluble form. The main functional domain of CMG-2 is an extracellular integrin-like von Willebrand factor type A (VWA) domain with a metal ion dependent adhesion site (MIDAS). This domain adheres selectively to collagen type IV and laminin (1 - 5). CMG-2 isoform 2 is induced in HUVEC as they undergo capillary formation in collagen matrices in vitro (3). CMG-2 is mutated in juvenile hyaline fibromatosis and infantile systemic hyalinosis disorders, and several of these mutations result in loss of laminin binding (6). CMG-2 and the related protein ATR/TEM8 serve as receptors for the protective antigen (PA) of Bacillus Anthracis (1, 2). After binding the VWA domain, PA undergoes furin-type cleavage, forms a heptameric receptor/PA pre-pore and binds LF or EF toxin subunits (5, 7, 8). Transport to low pH endosomes, which requires CMG-2 ubiquitination and interaction with the LDL receptor related protein LRP6 (9, 10), allows PA pore formation and release of toxin to the cytoplasm (10, 11). Soluble CMG-2 VWA domain acts as a dummy receptor that can protect cultured cells from anthrax intoxication (2). Within the extracellular region, human CMG-2 shares 84%, 81%,89% and 93% amino acid sequence homology with mouse, rat, bovine, and canine CMG-2, respectively. CMG-2 VWA domain also shares 60% aa identity with ATR/TEM8.

Item	Value
References	Scobie, H.M. and J.A.T. Young (2005) Curr. Opin. Microbiol. 8:106. Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170. Bell, S.E. et al. (2001) J. Cell Sci. 114:2755. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6367. Santelli, E. et al. (2004) Nature 430:905. Dowling, O. et al. (2003) Am. J. Hum. Genet. 73:957. Wigelsworth, D.J. et al. (2004) J. Biol. Chem. 279:23349. Go, M.Y. et al. (2006) J. Mol. Biol. 360:145. Abrami, L. et al. (2006) J. Cell Biol. 172:309. Wei, W. et al. (2006) Cell 124:1141. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13147.
Long Name	Capillary Morphogenesis Protein 2
Entrez Gene IDs	118429 (Human); 71914 (Mouse)
Alternate Names	ANTXR2, CMG-2, CMG-2MGC45856, CMG2, CMG2MGC111533, Capillary morphogenesis gene 2 protein, FLJ31074, ISH, JHF, JHS, anthrax toxin receptor 2, cI-35, capillary morphogenesis protein 2

Citations for Recombinant Human CMG-2 Protein, CF (1)

Showing 1 - 1 of 1 citation Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human CMG-2 Protein, CF include:

Species: N/A
Sample Types: N/A
Applications: Western Blot

Langer et al. (2012-01-01), Bacillus anthracis lethal toxin reduces human alveolar epithelial barrier function. Infect Immun, 80(12):4374-87.
PMID: 23027535

There are no citations that match your criteria.

Showing 1 - 1 of 1 citation Showing All

Product Documents for Recombinant Human CMG-2 Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Recombinant Human CMG-2 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased