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Recombinant Human CMG-2/ANTXR2 Fc Chimera Protein, CF

Catalog # 10360-AR | R&D Systems, Inc. a Bio-Techne Brand
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10360-AR-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CMG-2/ANTXR2 protein
Human CMG-2/ANTXR2
(Gln34-Asn317)
Accession # P58335.5
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln34 inferred from enzymatic pyroglutamate treatment revealing Glu35

Predicted Molecular Mass

57 kDa

SDS-PAGE

55-75 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Anthrax Protective Antigen is immobilized at 1.5 μg/mL (100 μL/well), it binds Recombinant Human CMG‑2/ANTXR2 Fc Chimera (Catalog # 10360-AR) with an ED50 of 1-6 ng/mL.

Scientific Data Images for Recombinant Human CMG-2/ANTXR2 Fc Chimera Protein, CF

Recombinant Human CMG-2/ANTXR2 Fc Chimera Protein Binding Activity

Recombinant Human CMG-2/ANTXR2 Fc Chimera Protein Binding Activity

When Anthrax Protective Antigen is immobilized at 1.5 μg/mL (100 μL/well), Recombinant Human CMG-2/ANTXR2 Fc Chimera (Catalog # 10360-AR) binds with an ED50 of 1-6 ng/mL.
Recombinant Human CMG-2/ANTXR2 Fc Chimera Protein SDS-PAGE

Recombinant Human CMG-2/ANTXR2 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CMG-2/ANTXR2 Fc Chimera (Catalog # 10360-AR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-75 kDa and 110-150 kDa, respectively.

Formulation, Preparation and Storage

10360-AR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CMG-2/ANTXR2

Capillary Morphogenesis Gene-2 (CMG-2) is a widely expressed anthrax toxin receptor (ATR) family protein (1 - 3). CMG-2 is a 55 kDa type I transmembrane (TM) protein that contains a 33 amino acid (aa) signal sequence, a 284 aa extracellular domain (ECD), a 24 aa TM segment, and a 147 aa cytoplasmic domain. There are three additional isoforms. Isoforms 4 shows a 12 aa insertion in the cytoplasmic region; isoform 2 shows a 103 aa deletion in the ECD; and isoform 3 is a truncated, 20 kDa, 289 aa soluble form. The main functional domain of CMG-2 is an extracellular integrin-like von Willebrand factor type A (VWA) domain with a metal ion dependent adhesion site (MIDAS). This domain adheres selectively to collagen type IV and laminin (1 - 5). CMG-2 isoform 2 is induced in HUVEC as they undergo capillary formation in collagen matrices in vitro (3). CMG-2 is mutated in juvenile hyaline fibromatosis and infantile systemic hyalinosis disorders, and several of these mutations result in loss of laminin binding (6). CMG-2 and the related protein ATR/TEM8 serve as receptors for the protective antigen (PA) of Bacillus Anthracis (1, 2). After binding the VWA domain, PA undergoes furin-type cleavage, forms a heptameric receptor/PA pre-pore and binds LF or EF toxin subunits (5, 7, 8). Transport to low pH endosomes, which requires CMG-2 ubiquitination and interaction with the LDL receptor related protein LRP6 (9, 10), allows PA pore formation and release of toxin to the cytoplasm (10, 11). Soluble CMG-2 VWA domain acts as a dummy receptor that can protect cultured cells from anthrax intoxication (2). Within the extracellular region, human CMG-2 shares 84%, 81%,89% and 93% amino acid sequence homology with mouse, rat, bovine, and canine CMG-2, respectively. CMG-2 VWA domain also shares 60% aa identity with ATR/TEM8.

References

  1. Scobie, H.M. and J.A.T. Young (2005) Curr. Opin. Microbiol. 8:106.
  2. Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170.
  3. Bell, S.E. et al. (2001) J. Cell Sci. 114:2755.
  4. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6367.
  5. Santelli, E. et al. (2004) Nature 430:905.
  6. Dowling, O. et al. (2003) Am. J. Hum. Genet. 73:957.
  7. Wigelsworth, D.J. et al. (2004) J. Biol. Chem. 279:23349.
  8. Go, M.Y. et al. (2006) J. Mol. Biol. 360:145.
  9. Abrami, L. et al. (2006) J. Cell Biol. 172:309.
  10. Wei, W. et al. (2006) Cell 124:1141.
  11. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13147.

Long Name

Capillary Morphogenesis Protein 2

Alternate Names

ANTXR2, cI-35, CMG2, ISH, JHS

Entrez Gene IDs

118429 (Human); 71914 (Mouse)

Gene Symbol

ANTXR2

UniProt

Product Documents for Recombinant Human CMG-2/ANTXR2 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CMG-2/ANTXR2 Fc Chimera Protein, CF

For research use only

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