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Recombinant Human CLF-1/CLC Complex Protein

Catalog # 1151-CL | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
1151-CL-025/CF

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With Carrier
1151-CL-025

Key Product Details

Source

NS0

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CLF-1/CLC Complex protein
Human CLF-1
(Ala38 - Arg422)
Accession # O75462.1
10-His tag
Human CLC
(Leu28 - Phe225)
Accession # Q9UBD9
6-His tag
N-terminus C-terminus

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala38 (CLF-1) & Leu28 (CLC)

Predicted Molecular Mass

44.5 kDa (CLF-1) & 23 kDa (CLC)

SDS-PAGE

50-65 kDa (CLF-1) & 28-30 kDa (CLC), reducing conditions

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells transfected with human CNTF R alpha.
The ED50 for this effect is 0.5‑3.5 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1151-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1151-CL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLF-1/CLC Complex

Cardiotrophin-like cytokine (CLC), also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3), is a member of the IL-6 family of cytokines (1, 2). CLC associates with the secreted soluble cytokine-like factor1 (CLF-1), a member of the Cytokine Type I Receptor family, to form the heteromeric composite cytokine CLC/CLF-1 (3, 5). CLC can also form an alternate composite cytokine with soluble ciliary neurotrophic factor receptor alpha (CNTF R alpha) (4). Co-expression of CLC with either CLF-1 or CNTF R alpha is required for the formation of the composite cytokines and for CLC secretion (3 - 5). CLC/CLF-1 binds to the membrane-associated CNTF R alpha to initiate the heterodimerization between gp130 and leukemia inhibitory factor receptor (LIFR) and stimulate thePI 3-kinase and the MAP kinase activity. The CLC/CLF-1 complex displays activities only on those cells expressing the functional tripartite receptor complex (5). Human and mouse CLF-1 share 96% amino acid sequence identity (3). Human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence homology. Human CLC also shares 96% amino acid sequence homology with mouse CLC. CLC/CLF-1 supports the survival of embryonic motor and sympathetic neurons and has been shown to induce astrocytes differentiation of fetal neuroepithelial cells (4 - 6). Both CLF-1 and CLC are expressed in the embryo, suggesting that the composite cytokine may have an important role in nervous system development (5, 7). CLC has also been shown to regulate immune functions by stimulating B cell proliferation and Ig production (8).

References

  1. Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.
  2. Shi, Y. et al. (1999) Biochem. Biophys. Res. Commun. 262:132.
  3. Elson, G.C.A. et al. (1998) J. Immunol. 161: 1371.
  4. Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
  5. Elson, G.C.A. et al. (2000) Nature Neurosci. 3:867.
  6. Uemura, A. et al. (2002) Cytokine 18:1.
  7. Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
  8. Senaldi, G. et al. (2002) J. Immunol. 168:5690.

Entrez Gene IDs

9244 (Human)

Gene Symbol

CRLF1

Product Documents for Recombinant Human CLF-1/CLC Complex Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CLF-1/CLC Complex Protein

For research use only

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