Recombinant Human CLEC-2/CLEC1B Fc Chimera Protein, CF

Catalog # 11211-CL | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

Q9P126.2

Source

CHO

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CLEC-2/CLEC1B protein
MD Human IgG1
(Pro100-Lys330)		 IEGR Human CLEC1B
(Gln58-Pro229)
Accession # Q9P126.2
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1 of Fc Chimera

Predicted Molecular Mass

47 kDa

SDS-PAGE

56-65 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CLEC-2/CLEC1B Fc Chimera (Catalog # 11211-CL) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human Podoplanin Fc Chimera binds with an ED50 of 6.00-60.0 pg/mL.

Scientific Data Images for Recombinant Human CLEC-2/CLEC1B Fc Chimera Protein, CF

Recombinant Human CLEC-2/CLEC1B Fc Chimera Protein Binding Activity.

When Recombinant Human CLEC-2/CLEC1B Fc Chimera (Catalog # 11211-CL) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human Podoplanin Fc Chimera binds with an ED50 of 6.00-60.0 pg/mL.

Recombinant Human CLEC-2/CLEC1B Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human CLEC-2/CLEC1B Fc Chimera Protein (Catalog # 11211-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 56-65 kDa and 110-130 kDa, respectively.

Formulation, Preparation and Storage

11211-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC-2/CLEC1B

C-type lectin-like receptor 2 (CLEC-2) is a 32 kDa, type II transmembrane glycoprotein and member of the C-type lectin-like family of receptors (1-4). CLEC-2 consists of a 33 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane region, and a 175 aa extracellular domain (SwissProt # Q9P126). The cytoplasmic domain contains multiple threonine and serine residues which are sites of potential phosphorylation, and a YXXL (Tyr-Xaa-Xaa-Leu) motif through which CLEC-2 does its signaling (2, 4-5). Ligand binding and cross-linking of CLEC-2 induces Src kinase-dependent tyrosine phosphorylation of the YXXL sequence, inducing activation of the tyrosine kinase Syk and initiation of a signaling pathway that culminates in activation of phospholipase C gamma2 (2, 5). The extracellular domain contains three potential sites of N-linked glycosylation, and a single carbohydrate recognition domain (CRD) which shows conservation of six cysteine residues (1, 6). Unlike most other members of the C-type lectin-like family of receptors, CLEC-2's CRD lacks the amino acid residues that are crucial for Ca2+-dependent carbohydrate binding, making it a non-classical C-type lectin receptor (1, 6). A splicing variant at aa 22-55 produces two isoforms for CLEC-2. Isoform 1 is the longer protein, and in isoform 2, an alanine residue is substituted for aa 22-55. Human CLEC-2 shares 63% aa sequence identity with mouse CLEC-2. CLEC-2 is expressed preferentially in liver, and is also detected in myeloid cells (monocytes, dendritic cells, and granulocytes) (1), platelets, and megakaryocytes (4). CLEC-2 is the receptor for the platelet-aggregating snake venom protein rhodocytin (3 - 4) and the molecule podoplanin, a transmembrane sialoglycoprotein that, when bound to CLEC-2, is involved in platelet aggregation, tumor metastasis, and lymphatic vessel formation (2, 7). CLEC-2 has also been shown to enhance infectivity of HIV-1 by mediating HIV-1 attachment and transfer by CLEC-2 transfected cells and platelets (8).

References

  1. Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
  2. Christou, C.M. et al. (2008) Biochem. J. 411:133.
  3. Watson, A.A. et al. (2007) J. Biol. Chem. 282:3165.
  4. Suzuki-Inoue, K. et al. (2006) Blood 107:542.
  5. Fuller, G.L. et al. (2007) J. Biol. Chem. 282:12397.
  6. Weis, W.I. et al. (1998) Immunol. Rev. 163:19.
  7. Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
  8. Chaipan, C. et al. (2006) J. Virol. 80:8951.

Long Name

C-type Lectin-like Receptor 2

Alternate Names

CLEC1B, CLEC2

Entrez Gene IDs

51266 (Human); 56760 (Mouse)

Gene Symbol

CLEC1B

UniProt

Q9P126.2

Product Documents for Recombinant Human CLEC-2/CLEC1B Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human CLEC-2/CLEC1B Fc Chimera Protein, CF

For research use only

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