Mouse myeloma cell line, NS0-derived human Chondrolectin protein Arg22-Asn216, with C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
34-40 kDa, under reducing conditions.
Measured by its binding ability in a functional ELISA. When Recombinant Human Chondrolectin His-tag Protein is immobilized at 1.00 ug/mL (100 µL/well), Recombinant Human IGFBP-5 binds with an ED50 of 5.00-30.0 ng/mL.
Recombinant Human Chondrolectin His-tag Protein, CF Scientific Data Examples
Recombinant Human Chondrolectin His-tag Protein Binding Activity.
When Recombinant Human Chondrolectin His-tag Protein (Catalog # 11059-CL) is immobilized at 1.00 ug/mL (100 µL/well), Recombinant Human IGFBP-5 (875-B5) binds with an ED50 of 5.00.0 ng/mL.
Recombinant Human Chondrolectin His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human Chondrolectin His-tag Protein (Catalog # 11059-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-40 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 1.00 mg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Chondrolectin (CHODL), also known as transmembrane protein MT75, is a type I transmembrane protein belonging to the C-type lectin-like domain superfamily. CHODL is predominantly expressed by the vascular muscle of the testis and smooth muscle of the heart and prostate (1) Mature CHODL consists of an extracellular domain (ECD) containing one C-type lectin carbohydrate recognition domain (CRD), a helical transmembrane domain, and a cytoplasmic tail. The ECD of CHODL shares 94% and 95% amino acid sequence identity with mouse and rat CHODL, respectively. Multiple isoforms of CHODL with varying C-terminal deletions are generated as a result of alternative splicing (2,3). CHODL is required for proper neuromuscular junction differentiation and is tightly regulated during early embryonic development (4). Dysregulation of CHODL expression is associated with spinal muscular atrophy in mouse (3). Overexpression of CHODL is associated with enhanced cell growth and invasion in lung cancer, while silencing of CHODL in colorectal cancer is a poor prognostic factor (5,6). Expression of specific CHODL isoforms is associated with T cell maturation (2).
Weng, L. et al. (2002) Genomics. 80:62.
Weng, L. et al. (2003) J. Biol. Chem. 278:19164.
Sleigh, J. et al. (2013) Hum. Mol. Genet. 23:855.
Oprişoreanu, A.M. et al. (2019) Cell Rep. 29:1082.
Masuda, K. et al. (2011) Clin. Cancer Res. 17:7712.
Zhang, X. et al. (2020) J. Cancer. 11:2874.
Entrez Gene IDs
140578 (Human); 246048 (Mouse)
C21orf68, CHODL, Chondrolectin, FLJ12627, MT75, PRED12, Transmembrane protein MT75, chromosome 21 open reading frame 68
Citations for Recombinant Human Chondrolectin His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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