Human embryonic kidney cell, HEK293-derived human CEACAM-20 protein Gln31-Gly450, with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Gln31 inferred from enzymatic pyroglutamate treatment revealing Leu32
Predicted Molecular Mass
73-84 kDa, under reducing conditions
Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. The ED50 for this effect is 0.25-1.5 μg/mL.
Recombinant Human CEACAM-20 His-tag Protein, CF Scientific Data Examples
Recombinant Human CEACAM-20 His-tag Protein Bioactivity
Immobilized Recombinant Human CEACAM-20 His-tag (Catalog # 10277-CM) enhances L cells mouse fibroblast cell line adhesion. The ED50 for this effect is 0.25‑1.5 μg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carcinoembryonic antigen-related cell adhesion molecule-20 (CEACAM-20) is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. Mature human CEACAM-20 consists of a 420 amino acid (aa) extracellular domain (ECD), a 21 aa helical transmembrane segment, and a 114 aa cytoplasmic domain. The extracellular domain possesses four IgC2-like domains which are stabilized by disulfide bonds, as well as several predicted glycosylation sites (1-5). The extracellular domain of CEACAM-20 is also unique among the CEACAMs because it contains a truncated IgV-like N domain (2). Within the ECD, human CEACAM-20 shares 64% and 62% aa identity with the mouse and rat CEACAM-20, respectively. The cytoplasmic domain is unusually long compared to most other CEACAMs and is predicted to contain four tyrosine phosphorylation sites, two of which correspond to the immune-receptor tyrosine-based activation motif (ITAM) (2, 3). Human CEACAM proteins have been linked to numerous intercellular-adhesion and intracellular signaling processes including cell adhesion, growth, and recognition, differentiation, angiogenesis, and apoptosis (7, 8). Human CEACAM-20 expression is limited to the reproductive system and the intestinal tract, with the highest levels of expression found in the small intestine and prostate (2, 3). An in vitro model of human prostate morphogenesis showed that CEACAM-20 is co-expressed with CEACAM-1 and plays a critical role in the formation of prostate organoids, making it a marker for prostate cancer (2). Although the exact mechanism is not fully understood, CEACAM-20 may promote the proliferation of intestinal epithelial cells (IECs) (9). There is evidence suggesting CEACAM-20 can induce the production of chemokines like interleukin (IL)-8 and stimulate inflammatory responses in colitis and Crohn's disease (6). CEACAM-20 is also thought to act as a physiological substrate for SAP-1 in the intestinal epithelium (10).
Tchoupa, A. et al. (2014) J Cell Commun Signal 12:27.
Zhang, H. et al. (2013) PLoS ONE 8:e53359.
Zebhauser, R. et al. (2005) Genomics 86:566.
Beauchemin, N. Arabzadeh, A. (2013) Cancer Metastasis Rev 32(3):643.
Kuespert, K. et al. (2006) Curr Opin Cell Biol 18:565.
Murata, Y. et al. (2015) PNAS E4264.
Obrink, B. (1997) Curr Opin Cell Biol 9:616.
Horst, AK. Wagener, C. (2004) Handb Exp Pharmacol 283.
Kitamura, Y. et al. (2015) Genes to Cells 20:578.
Kotani, T. et al. (2016) Expert Review of Gastroenterology & Hepatology. 10:1313.
Which Brands are Currently Available on bio-techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments and Simple Plex Assays are available to quote. ProteinSimple branded instrument consumables and ACD branded products will be available on bio-techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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