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Recombinant Human CEACAM-1/CD66a Protein, CF

Bio-Techne includes R&D Systems | Catalog # 2244-CM

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2244-CM-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CEACAM-1/CD66a protein
Gln35-Gly428, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln35

Predicted Molecular Mass

44.6 kDa

SDS-PAGE

100-130 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-2 secretion by HuT 78 human cutaneous T cell lymphoma cells Chen, C.J. and J.E. Shively (2004) J. Immunol. 172:3544.
The ED50 for rhCEACAM-1 inhibition of anti-CD3 induced IL-2 production is 50.0-200 ng/mL.

Formulation, Preparation and Storage

2244-CM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-1/CD66a

Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM-1; also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1 - 3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I transmembrane proteins (CEACAM-1, 3 & 4) and GPI-linked molecules (CEACAM-5-8). There is no CEACAM-2 in human. The gene for human CEACAM-1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74 aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type Ig-like domain, followed by three C2-type Ig-like domains. It shows considerable glycosylation, including high mannose residues and (sialyl) LewisX (1). The cytoplasmic region shows one ITIM motif and a calmodulin binding site (1 - 3). In addition to the full length form, ten alternate splice forms have been reported (1, 4, 6, 7, 8). There are three soluble and seven transmembrane isoforms, with variations occurring in both the ECD and cytoplasmic region. All ten alternate splice forms contain the V-type Ig-like domain (aa’s 35 - 142). The three soluble forms also contain the first two C2-type Ig-like domains (aa’s 145 - 317), with differences coming in the third C2-type Ig-like domain (6). The seven transmembrane isoforms are highly divergent. Five of the seven contain the V-type plus the first two C2-type domains and then diverge considerably both in the ECD and cytoplasmic region. The remaining two contain only the V-type Ig-like domain, the transmembrane region, and either a full-length or truncated cytoplasmic tail (1, 8). The actual functions of the isoforms are unclear.
Full-length mouse and rat CEACAM-1 are approximately 57% aa identical to human CEACAM-1; in the V-type Ig-like domain, they are 58% and 56% aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium. It is known to act as an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEA and CEACAM-6/NCA (3, 9). On neutrophils, CEACAM-1 also binds to dendritic cell CD-SIGN via its LeX moiety, inducing dendritic cell maturation and a subsequent Th1-type response (10,11).

References

  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Thompson, J. et al. (1992) Genomics 12:761.
  3. Waggener, C. and S. Ergun (2000) Exp. Cell Res. 261:19.
  4. Barnett, T.R. et al. (1989) J. Cell Biol. 108:267.
  5. Hinoda, Y. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6959.
  6. Kuroki, M. et al. (1991) Biochem. Biophys. Res. Commun. 176:578.
  7. Barnett, T.R. et al. (1993) Mol. Cell. Biol. 13:1273.
  8. Watt, S.M. et al. (1994) Blood 84:200.
  9. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
  10. Klaas, P.J.M. et al. (2005) FEBS Lett. 579:6159.
  11. Bogoevska, V. et al. (2005) Glycobiology 16:197.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 1

Alternate Names

BGP1, Biliary Glycoprotein 1, CD66a, Cea-1, CEACAM1, Hv-1, Hv-2, MHVR

Entrez Gene IDs

634 (Human); 26365 (Mouse); 102139857 (Cynomolgus Monkey)

Gene Symbol

CEACAM1

UniProt

Additional CEACAM-1/CD66a Products

Product Documents for Recombinant Human CEACAM-1/CD66a Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CEACAM-1/CD66a Protein, CF

For research use only

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