Recombinant Human CDCP1 (Secreted Form) Fc Protein, CF

Name: Recombinant Human CDCP1 (Secreted Form) Fc Protein, CF
Brand: R&D Systems
SKU: 10328-CU

Catalog # 10328-CU

Product Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	HEK293
Accession #:	Q9H5V8-3
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human CDCP1 (Secreted Form) Fc Protein, CF

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CDCP1 protein

Human CDCP1 (Phe30-Glu343) Accession # Q9H5V8-3	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Phe30

Predicted Molecular Mass

62 kDa

SDS-PAGE

75-95 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CDCP1 Fc Chimera (Catalog # 10328-CU) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human P‑Cadherin Fc Chimera (Catalog # 861-PC) binds with an ED₅₀ of 0.75-6 μg/mL.

Recombinant Human CDCP1 (Secreted Form) Fc Protein, CF Scientific Data Examples

	Recombinant Human CDCP1 (Secreted Form) Fc Protein Binding Activity When Recombinant Human CDCP1 Fc Chimera (Catalog # 10328-CU) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human P-Cadherin Fc Chimera (Catalog # 861-PC) binds with an ED₅₀ of 0.75-6 µg/mL.
	Recombinant Human CDCP1 (Secreted Form) Fc Protein SDS-PAGE 2 μg/lane of Recombinant Human CDCP1 (Secreted Form) Fc Chimera (Catalog # 10328-CU) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-95 kDa and 150-190 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10328-CU

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CDCP1

CDCP1 (CUB-domain containing protein 1), also known as Trask or CD318, is a type I transmembrane receptor whose specific role remains unclear. Human CDCP1 is synthesized with a large extracellular domain (ECD) containing three CUB domains, a transmembrane domain and a cytoplasmic domain with 5 tyrosine phosphorylation sites (1). Full-length CDCP1 is ~135 kDa and can be cleaved into a ~70 kDa membrane-bound and a ~65 kDa circulating form (2). A secreted only form of human CDCP1, also called Isoform 2, can also be expressed consisting of amino acids (aa) 30-343 of the ECD (3). Mature human Isoform 2 shares 86% and 85% aa sequence identity to mouse and rat CDCP1 Isoform 2, respectively. CDCP1 was originally identified from proteins involved in metastasis and has been found on tumor, stem cells, keratinocytes and colonic epithelial cells (1). Tyrosine phosphorylation of the intracellular domain of CDCP1 results in downstream signaling through Src-family kinases (SFKs), Akt, and PKCδ (2, 4, 5). CDCP1 appears to be important for cell-cell and cell-substratum adhesion and the phosphorylation state of CDCP1 regulates this effect (5, 7). CDCP1 is associated with a poor prognosis in epithelial tumors, such as lung, pancreatic, colorectal, renal, and ovarian carcinomas (rEf) and is a novel marker of the most aggressive human triple-negative breast cancers (7, 8). Several different extracellular forms of CDCP1 have been shown to exhibit disease-specific expression in prostate cancer (9).

Item	Value
References	Hooper, J.D. et al. (2003) Oncogene 22:1783. He, Y. et al. (2010) J. Biol. Chem. 285:26162. Perry, S.E. (2007) FEBS Lett. 581:1137. Law, M.E. et al. (2016) Breast Cancer Res. 18:80. Casar B. et al. (2012) Oncogene 31:3924. Spassov, D.S. et al. (2013) Cancer Res. 73:1168. Turdo, F. et al. (2016) Oncotarget 7:69649. Wright, H.J. et al. (2016) Oncogene 35:4762. Yang, L. et al. (2015) Oncotarget 6:43743.
Long Name	CUB Domain Containing Protein 1
Entrez Gene IDs	64866 (Human); 109332 (Mouse)
Alternate Names	CD318, CD318 antigen, CDCP1, CUB domain containing protein 1, CUB domain-containing protein 1, Membrane glycoprotein gp140, SIMA135, SIMA135TRASK, Subtractive immunization M plus HEp3-associated 135 kDa protein, Transmembrane and associated with src kinases

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