Recombinant Human CD94 Fc Chimera Protein, CF

Name: Recombinant Human CD94 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10281-CD

Catalog # 10281-CD

Product Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	HEK293
Accession #:	Q13241.2
Structure / Form:	Disulfide-linked homodimer.
Applications:	Bioactivity

View Full Technical Details for Recombinant Human CD94 Fc Chimera Protein, CF

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD94 protein

MD	Human IgG₁ (Pro100-Lys330)	IEGR	Human CD94 (Lys32-Ile179) Accession # Q13241.2
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Met & Asp & Pro100

Predicted Molecular Mass

44 kDa

SDS-PAGE

53-62 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human NKG2E Fc Chimera (Catalog # 9759-NK) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human CD94 Fc Chimera (Catalog # 10281-CD) binds with an ED₅₀ of 1.25-10 µg/mL.

Recombinant Human CD94 Fc Chimera Protein, CF Scientific Data Examples

	When Recombinant Human NKG2E Fc Chimera (9759-NK) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human CD94 Fc Chimera (Catalog # 10281-CD) binds with an ED₅₀ of 1.25-10 µg/mL.
	2 μg/lane of Recombinant Human CD94 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-62 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10281-CD

Formulation	Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD94

CD94 is an approximately 25 kDa type 2 transmembrane protein that plays an important role in regulating natural killer (NK) cell activation (1). Human CD94 consists of a 10 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 148 aa extracellular domain (ECD) with a stem region and one C-type lectin domain (2). Alternative splicing generates additional isoforms that lack either the stem region, the terminal half of the ECD, or the cytoplasmic and transmembrane regions (3). Within the ECD, human CD94 shares 53% and 55% amino acid identity to mouse and rat CD94, respectively. CD94 is expressed at varying cell surface density on NK cells during their differentiation and on a subset of CD8⁺ T cells (4). It associates into disulfide-linked heterodimers with NKG2A/B, C, or E (5-8), and these complexes function as receptors for the nonclassical MHC class I molecule, HLA-E (9, 10). Ligation of CD94-NKG2A or CD94-NKG2C on NK cells triggers inhibitory or activating signals, respectively (11).

Item	Value
References	Chester, C. et al. (2015) Front. Immunol. 6:601. Chang, C. et al. (1995) Eur. J. Immunol. 25:2433. Lieto, L.D. et al. (2006) Genes Immun. 7:36. Yu, J. et al. (2010) Blood 115:274. Phillips, J.H. et al. (1996) Immunity 5:163. Lazetic, S. et al. (1996) J. Immunol. 157:4741. Brooks, A.G. et al. (1997) J. Exp. Med. 185:795. Carretero, M. et al. (1997) Eur. J. Immunol. 27:563. Braud, V.M. et al. (1998) Nature 391:795. Kraemer, T. et al. (2014) J. Immunol. Res. 2014:352160. Houchins, J.P. et al. (1997) J. Immunol. 158:3603.
Entrez Gene IDs	3824 (Human); 16643 (Mouse); 102119829 (Cynomolgus Monkey)
Alternate Names	CD94, CD94 antigen, CD94natural killer cells antigen CD94, KLRD1, KP43, Killer cell lectin-like receptor subfamily D member 1, NK cell receptor, killer cell lectin-like receptor subfamily D, member 1

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