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Recombinant Human CD94 Fc Chimera Protein, CF

Catalog # 10281-CD | R&D Systems, Inc. a Bio-Techne Brand
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10281-CD-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer.

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD94 protein
MD Human IgG1
(Pro100-Lys330)
IEGR Human CD94
(Lys32-Ile179)
Accession # Q13241.2 
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met & Asp & Pro100

Predicted Molecular Mass

44 kDa

SDS-PAGE

53-62 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human NKG2E Fc Chimera (Catalog # 9759-NK) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human CD94 Fc Chimera (Catalog # 10281-CD) binds with an ED50 of 1.25-10 µg/mL.

Scientific Data Images for Recombinant Human CD94 Fc Chimera Protein, CF

When Recombinant Human NKG2E Fc Chimera (9759-NK) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human CD94 Fc Chimera (Catalog # 10281-CD) binds with an ED50 of 1.25-10 µg/mL.
2 μg/lane of Recombinant Human CD94 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-62 kDa.

Formulation, Preparation and Storage

10281-CD
Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: CD94

CD94 is an approximately 25 kDa type 2 transmembrane protein that plays an important role in regulating natural killer (NK) cell activation (1). Human CD94 consists of a 10 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 148 aa extracellular domain (ECD) with a stem region and one C-type lectin domain (2). Alternative splicing generates additional isoforms that lack either the stem region, the terminal half of the ECD, or the cytoplasmic and transmembrane regions (3). Within the ECD, human CD94 shares 53% and 55% amino acid identity to mouse and rat CD94, respectively. CD94 is expressed at varying cell surface density on NK cells during their differentiation and on a subset of CD8+ T cells (4). It associates into disulfide-linked heterodimers with NKG2A/B, C, or E (5-8), and these complexes function as receptors for the nonclassical MHC class I molecule, HLA-E (9, 10). Ligation of CD94-NKG2A or CD94-NKG2C on NK cells triggers inhibitory or activating signals, respectively (11).

References

  1. Chester, C. et al. (2015) Front. Immunol. 6:601.
  2. Chang, C. et al. (1995) Eur. J. Immunol. 25:2433.
  3. Lieto, L.D. et al. (2006) Genes Immun. 7:36.
  4. Yu, J. et al. (2010) Blood 115:274.
  5. Phillips, J.H. et al. (1996) Immunity 5:163.
  6. Lazetic, S. et al. (1996) J. Immunol. 157:4741.
  7. Brooks, A.G. et al. (1997) J. Exp. Med. 185:795.
  8. Carretero, M. et al. (1997) Eur. J. Immunol. 27:563.
  9. Braud, V.M. et al. (1998) Nature 391:795.
  10. Kraemer, T. et al. (2014) J. Immunol. Res. 2014:352160.
  11. Houchins, J.P. et al. (1997) J. Immunol. 158:3603.

Alternate Names

CD94, KLRD1

Entrez Gene IDs

3824 (Human); 16643 (Mouse); 102119829 (Cynomolgus Monkey)

Gene Symbol

KLRD1

UniProt

Product Documents for Recombinant Human CD94 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CD94 Fc Chimera Protein, CF

For research use only

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