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Recombinant Human CD90/Thy1 His-tag Protein, CF

Catalog # 10805-TH | R&D Systems, Inc. a Bio-Techne Brand
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10805-TH-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD90/Thy1 protein
Gln20-Ser141, with a C-terminal 6-His tag. Accession # P04216.2

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln20 inferred from enzymatic pyroglutamate treatment revealing Lys21.

Predicted Molecular Mass

14 kDa

SDS-PAGE

19-33 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD90/Thy-1 His-tag is immobilized at 5.00 µg/mL (100 µL/well), Recombinant Human Galectin-1 (Catalog # 1152-GA) binds with an ED50 of 0.400-2.00 μg/mL.

Scientific Data Images for Recombinant Human CD90/Thy1 His-tag Protein, CF

Recombinant Human CD90/Thy1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human CD90/Thy1 His-tag Protein (Catalog # 10805-TH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 19-33 kDa.

Formulation, Preparation and Storage

10805-TH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD90/Thy1

CD90, also known as Thy1, is an approximately 25-35 kDa glycoprotein that mediates cellular adhesion and exerts wide ranging effects in different tissues (1, 2). CD90 is expressed on hematopoietic progenitor cells, neurons, and activated vascular endothelial cells (3-5). Its species-specific expression in lymphoid cells allows its use as a thymocyte and pan T cell marker in mouse but not in human (1). CD90 can form dimers and higher order multimers (6). It binds to heparin, the proteoglycan Syndecan-4, and Integrins alpha M beta 2, alpha V beta 3, and alpha V beta 5 (5, 7-10). Through these interactions, CD90 inhibits neurite outgrowth (4), promotes astrocyte adhesion (7), mediates the adhesion and extravasation of leukocytes and melanoma cells (5, 9), supports the Thrombospondin-1 induced disassembly of fibroblast focal adhesions (11), and inhibits the activation of latent TGF-beta 1, myofibroblast differentiation, and lung fibrosis (10). Human Thy-1 shares 63% and 66% amino acids similarity with mouse and rat homolog.

References

  1. Haeryfar, S.M.M. and D.W. Hoskin (2004) J. Immunol. 173:3581.
  2. Rege, T.A. and J.S. Hagood (2006) Biochim. Biophys. Acta 1763:991.
  3. Craig, W. et al. (1993) J. Exp. Med. 177:1331.
  4. Tiveron, M-C. et al. (1992) Nature 355:745.
  5. Wetzel, A. et al. (2004) J. Immunol. 172:3850.
  6. Mahanthappa, N.K. and P.H. Patterson (1992) Dev. Biol. 150:60.
  7. Avalos, A.M. et al. (2009) J. Cell Sci. 122:3462.
  8. Hueber, A.O. et al. (1992) J. Immunol. 148:3692.
  9. Saalbach, A. et al. (2005) Oncogene 24:4710.
  10. Zhou, Y. et al. (2010) J. Biol. Chem. 285:22382.
  11. Barker, T.H. et al. (2004) J. Biol. Chem. 279:23510.

Alternate Names

CD90, Thy1

Entrez Gene IDs

7070 (Human); 21838 (Mouse)

Gene Symbol

THY1

UniProt

Product Documents for Recombinant Human CD90/Thy1 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CD90/Thy1 His-tag Protein, CF

For research use only

Reconstitution Calculator

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