Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Recombinant Human CD8 alpha Fc Chimera Protein, CF

Catalog # 10927-CD | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
10927-CD-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD8 protein
Human CD8 alpha
(Ser22-Asp182)
Accession # P01732.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser22

Predicted Molecular Mass

44 kDa

SDS-PAGE

54-60 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Human CD8 alpha Antibody (Catalog # MAB3803) is immobilized at 2.00 μg/mL, 100 μg/mL, Recombinant Human CD8 alpha alpha Fc Chimera binds with an ED50 of 1.50-15.0 ng/mL.

Scientific Data Images for Recombinant Human CD8 alpha Fc Chimera Protein, CF

Recombinant Human CD8 alpha Fc Chimera Protein Binding Activity.

When Human CD8a Antibody (MAB3803) is immobilized at 2 μg/mL, 100 μg/mL, Recombinant Human CD8a Fc Chimera Protein (Catalog # 10927-CD) binds with an ED50 of 1.50-15.0 ng/mL.

Recombinant Human CD8 alpha Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human CD8 alpha Fc Chimera Protein (Catalog # 10927-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-60 kDa and 110-120 kDa, respectively.

Formulation, Preparation and Storage

10927-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8, also known as Ly2 or Leu2, is a heterodimeric glycoprotein (alpha and beta subunits) that functions in conjunction with the T cell receptor in the recognition of MHC class I/peptide complexes (1, 2). CD8 alpha is expressed on double positive (CD4+ CD8+) thymocytes and mature CD8+ cytolytic T cells (CTL) (3-5), intraepithelial lymphocytes (IEL) (6), some gamma delta T cells (7), subsets of thymic and splenic dendritic cells (DC) (88), and peritoneal mast cells (9). It can form disulfide linked homodimers or heterodimers with CD8 beta (10). Thymic CD8+ DC express primarily alpha beta heterodimers, while splenic CD8+ DC primarily express alpha alpha homodimers (8). CD8a+ DC can present viral antigenic peptides in complex with MHC I and prime CTL responses (11). The approximately 35 kDa mature mouse CD8 alpha consists of a 169 amino acid (aa) extracellular domain (ECD) with one Iglike domain, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (12). Within the ECD, mouse CD8 alpha shares 49% and 64% aa sequence identity with human and rat CD8 alpha, respectively.

References

  1. Laugel, B. et al. (2011) J. Leukoc. Biol. 90:1089.
  2. Cole, D.K. et al. (2012) Immunology 137:139.
  3. Germain, R.N. (2002) Nat. Rev. Immunol. 2:309.
  4. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  5. Nakayama, K. et al. (1994) Science 263:1131.
  6. Wang, J. and J.R. Klein (1994) Science 265:1860.
  7. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
  8. Vremec, D. et al. (1992) J. Exp. Med. 176:47.
  9. Lin, T.J. et al. (1998) J. Immunol. 161:6265.
  10. Snow, P.M. and C. Terhorst (1983) J. Biol. Chem. 258:14675.
  11. Belz, G.T. et al. (2004) J. Immunol. 172:1996.
  12. Nakauchi, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:5126.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

UniProt

Additional CD8 Products

Product Documents for Recombinant Human CD8 alpha Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CD8 alpha Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Loading...
Setting the standard in quality research reagents