Product Specifications for Recombinant Human CD5L Protein, CF
Mouse myeloma cell line, NS0-derived human CD5L protein Ser20-Gly347, with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
43 kDa, reducing conditions
Measured by its ability to inhibit apoptosis on the J774A.1 mouse reticulum cell sarcoma macrophage cells. Miyazaki, T. et al. (1999) J. Exp. Med. 189:413. The presence of rhCD5L inhibited rhFas Ligand induced apoptosis by 70-90%. The ED50 for this effect is 1-4 µg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
CD5L (CD5 antigen-like), also known as Sp alpha and AIM, is a 50 kDa secreted glycoprotein that belongs to the SRCR (scavenger receptor cysteine rich) group B family of proteins. Group B proteins are distinguished by SRCR domains that are encoded by a single exon (1 - 3). The human CD5L cDNA encodes a 347 amino acid (aa) precursor that includes a 19 aa signal sequence and three SRCR domains (4, 5). Among group B proteins, CD5L is most closely related to CD5 and CD6, with which it shares 18% and 31% aa sequence identity, respectively. CD5L is up-regulated in macrophages at inflammatory sites. It sustains inflammatory reactions by both increasing the phagocytic capacity of macrophages and impeding the apoptosis of local macrophages, NK cells, and T cells (6, 7). Agonists of the LXR and RXR nuclear hormone receptors induce CD5L upregulation in macrophages and reduce macrophage apoptosis (8, 9). Oxidized LDL (which acts through LXR/RXR) is taken up by macrophages, promoting their development into foam cells. The increased level of CD5L protects foam cells from apoptosis but permits more rapid cellular accumulation and atherosclerotic plaque formation (9). In activated B cells, however, the combination of CD5L and TGF-beta inhibits proliferation. The binding of CD5L to splenic B cells is increased following TGF-beta exposure, suggesting that TGF-beta increases the expression or availability of an unidentified CD5L receptor (5, 10). CD5L also functions as a pattern recognition molecule by binding both lipoteichoic acid on Gram positive and lipopolysaccharide on Gram negative bacteria (11). In the thymic cortex, CD5L protects cortical CD4+CD8+ thymocytes from apoptosis (12). CD5L circulates in the serum in complex with IgM (13).
Sarrias, M.R. et al. (2004) Crit. Rev. Immunol. 24:1.
Resnick, D. et al. (1994) Trends Biochem. Sci. 19:5.
Mukhopadhyay, S. and S. Gordon (2004) Immunobiology 209:39.
Gebe, J.A. et al. (1997) J. Biol. Chem. 272:6151.
Gebe, J.A. et al. (2000) Immunol. 99:78.
Haruta, I. et al. (2001) J. Biol. Chem. 276:22910.
Kuwata, K. et al. (2003) Am. J. Pathol. 162:837.
Valledor, A.F. et al. (2004) Proc. Natl. Acad. Sci. 101:17813.
Arai, S. et al. (2005) Cell Metab. 1:201.
Yusa, S. et al. (1999) Eur. J. Immunol. 29:1086.
Sarrias, M-R. et al. (2005) J. Biol. Chem. 280:35391.
Miyazaki, T. et al. (1999) J. Exp. Med. 189:413.
Tissot, J.D. et al. (2002) Electrophoresis 23:1203.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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