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Recombinant Human CD46 Fc Chimera Protein, CF

Catalog # 10257-CD | R&D Systems, Inc. a Bio-Techne Brand
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10257-CD-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD46 protein
Human CD46
(Cys35-Asp328)
Accession # P15529-11
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys35

Predicted Molecular Mass

59 kDa

SDS-PAGE

88-98 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD46 Fc Chimera is immobilized at 5 μg/mL (100 μL/well), the concentration of Human C4b that produces 50% of the optimal binding response is 2-12 μg/mL.

Scientific Data Images for Recombinant Human CD46 Fc Chimera Protein, CF

Recombinant Human CD46 Fc Chimera Protein Binding Activity

Recombinant Human CD46 Fc Chimera Protein Binding Activity

When Recombinant Human CD46 Fc Chimera (Catalog # 10257-CD) is immobilized at 5 μg/mL (100 μL/well), it binds to Human C4b with an ED50 of 2‑12 μg/mL.
Recombinant Human CD46 Fc Chimera Protein SDS-PAGE

Recombinant Human CD46 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CD46 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 88-98 kDa and 160-190 kDa, respectively.

Formulation, Preparation and Storage

10257-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD46

CD46, also known as MCP (Membrane cofactor protein), is a type I membrane protein that functions as a cofactor for Complement Factor I­mediated inactivation of complement components C3b and C4b (1, 2). Mature human CD46 consists of a 309 amino acid (aa) extracellular domain (ECD) with four short consensus repeats (SCR) and a Ser/Thr/Pro-enriched region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain (1). Within the ECD, human CD46 shares 50% aa sequence identity with mouse and rat CD46. CD46 is commonly expressed in four distinct isoforms, and all isoforms have conserved complement control protein domains which harbor the C3b and C4b binding sites (3). CD46 is expressed on all nucleated cells (4). CD46 participates in the maintenance of immune homeostasis and the induction of protective T cell immunity, and it could extend towards key processes in development and cell metabolism (5).

References

  1. Liszewski, M.K. et al. (1991) Annu. Rev. Immunol. 9:431.
  2. Barilla-LaBarca, M.L. et al. (2002) J. Immunol. 168:6298.
  3. Yamamoto, H. et al. (2013) Intl. J. Biochem. Cell Biol. 45:2808.
  4. Andrews, P.W. et al. (1985) Ann. Hum. Genet. 49:31.
  5. Kolev, M. et al. (2013) Semin. Immunol. 25:12.

Alternate Names

CD46, MCP

Entrez Gene IDs

4179 (Human); 17221 (Mouse)

Gene Symbol

CD46

UniProt

Product Documents for Recombinant Human CD46 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CD46 Fc Chimera Protein, CF

For research use only

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