Recombinant Human CD44v5 His-tag Protein, CFNew

Name: Recombinant Human CD44v5 His-tag Protein, CF
Brand: R&D Systems
SKU: 11087-CD

Catalog # 11087-CD

Product Datasheet / COA / SDS

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Key Product Details

Source:	CHO
Accession #:	NP_001001391.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human CD44v5 His-tag Protein, CF

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD44 protein

Human CD44v5 (Gln21-Thr222) NVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHST (Arg223-Trp269) Accession # NP_001001391.1	6-His tag
N-terminus	C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gln21

Predicted Molecular Mass

32 kDa

SDS-PAGE

76-86 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD44v5 His-tag Protein is immobilized at 2.00 µg/mL (100 µL/well), Recombinant Human LSECtin/CLEC4G (Catalog # 2947-CL) binds with an ED₅₀ of 5.00-30.0 ng/mL.

Recombinant Human CD44v5 His-tag Protein, CF Scientific Data Examples

	Recombinant Human CD44v5 His-tag Protein Binding Activity. When Recombinant Human CD44v5 His-Tag Protein (Catalog # 11087-CD) is immobilized at 2.00 µg/mL (100 µL/well), Recombinant Human LSECtin/CLEC4G (2947-CL) binds with an ED₅₀ of 5.00-30.0 ng/mL.
	Recombinant Human CD44v5 His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human CD44v5 His-tag Protein (Catalog # 11087-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 76-86 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11087-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

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Desired Reconstitution Concentration

Background: CD44

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1‑3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan‑binding disulfide‑stabilized link region and a 325‑530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 transcripts undergo complex alternative splicing, and, within the stem, ten variably spliced exons (v1‑10 corresponding to exons 6‑15; although human CD44 lacks v1/exon 6) produce multiple protein isoforms (1‑4). The standard or hematopoietic form, CD44H, does not include the variable segments (1‑4). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 4, 5). Human CD44v5 contains exon 10 and is associated with tumor progression and metastasis in many types of cancer including breast, colon, lung, renal, skin, and ovarian tumors (6, 12). With variable N‑ and O‑glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N‑terminal invariant portion of the ECD (aa 21‑222), human CD44 shares 76%, 76%, 86%, 83% and 79% identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a "platform" for growth factors and metalloproteinases. Second, CD44 can function as a co‑receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (6) or may be cleaved at multiple sites by either membrane‑type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (8, 9). The cellular portion may then undergo gamma secretase‑dependent intramembrane cleavage to form an A beta‑like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (10, 11). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 8). CD44v5 plays important role in cancer progression involving in cell migration and invasion and is considered a functional cancer biomarker (6, 12).

Item	Value
References	Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33. Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160. Screaton, G.R. et al. (1993) J. Biol. Chem. 268:12235. Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931. Todaro, M. et al. (2014) Cell stem cell 14:342 Vizoso, F.J. et al. (2004) J. Cancer Res. Clin. Oncol. 130:679. Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930. Nakamura, H. et al. (2004) Cancer Res. 64:876. Murakami, D. et al. (2003) Oncogene 22:1511. Lammich, S. et al. (2002) J. Biol. Chem. 277:44754. Fox, S.B. et al. (1994) Cancer Res. 54:4539.
Entrez Gene IDs	960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
Alternate Names	CD44, CD44 antigen, CD44 molecule (Indian blood group), CD44R, CDw44, CSPG8, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HCAM, HCELL, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU2CD44 antigen (homing function and Indian blood group system), MDU3, MDU3CDW44, MIC4, MIC4MGC10468, MUTCH-I, PGP-1, PGP-I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, hematopoietic cell E- and L-selectin ligand, homing function and Indian blood group system

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