Source	Chinese Hamster Ovary cell line, CHO-derived human CD4 protein Lys26-Trp390
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Lys26
Predicted Molecular Mass	41 kDa
SDS-PAGE	48-52 kDa, under reducing conditions
Activity	Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. The ED₅₀ for this effect is 0.75-4.5 μg/mL.

Recombinant Human CD4 Protein, CF Scientific Data Examples

	Recombinant Human CD4 Protein Bioactivity Immobilized Recombinant Human CD4 Protein (Catalog # 10327-CD) supports the adhesion of HeLa human cervical epithelial carcinoma cells. The ED₅₀ for this effect is 0.75-4.5 μg/mL.
	Recombinant Human CD4 Protein SDS-PAGE 2 μg/lane of Recombinant Human CD4 (Catalog # 10327-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-52 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10327-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD4

CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations (1). Mature human CD4 consists of a 371 amino acid (aa) extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain (2). Within the ECD, human CD4 shares approximately 52% aa sequence identity with mouse and rat CD4. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4⁺ or CD8⁺ mature T cells (3). CD4⁺ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4⁺ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (4). CD4 is reexpressed on circulating CD8⁺ T cells upon activation and contributes to their cytotoxic effector activity (5). In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain (6-9). Similar CD4 distribution between species cannot be assumed as demonstrated by its presence on macrophages in human and rat but not in mouse (6). CD4 binds directly to MHC class II molecules on antigen presenting cells (10). This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction (1, 11). Palmitoylation of two cysteine residues in the cytoplasmic tail of CD4 promotes the localization of CD4 in lipid rafts and its ability to augment TCR signaling via activation of the tyrosine kinase Lck (12). CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co-receptor for the gp120 surface glycoprotein of HIV-1 (7, 13-15).

Item	Value
References	Vignali, D.A.A. (2010) J. Immunol. 184:5933. Maddon, P.J. et al. (1985) Cell 42:93. Alarcon, B. and H.M. van Santen (2010) Sci. Signal. 3:pe11. Wan, Y.Y. and R.A. Flavell (2009) Mol. Cell Biol. 1:20. Kitchen, S.G. et al. (2005) Proc. Natl. Acad. Sci. 102:3794. Crocker, P.R. et al. (1987) J. Exp. Med. 166:613. Biswas, P. et al. (2003) Blood 101:4452. Bernstein, H.B. et al. (2006) J. Immunol. 177:3669. Funke, I. et al. (1987) J. Exp. Med. 165:1230. Doyle, C. and J.L. Strominger (1987) Nature 330:256. Huppa, J.B. et al. (2010) Nature 463:963. Fragoso, R. et al. (2003) J. Immunol. 170:913. Cruikshank, W.W. et al. (1994) Proc. Natl. Acad. Sci. 91:5109. Klatzmann, D. et al. (1984) Nature 312:767. Dagleish, A.G. et al. (1984) Nature 312:763.
Entrez Gene IDs	920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names	CD4, CD4 antigen, CD4 antigen (p55), CD4 molecule, CD4 receptor, CD4mut, CD_antigen: CD4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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Recombinant Human CD4 Protein, CF

Name: Anonymous

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Verified Customer | Posted 02/08/2021

Octet binding assay. Immobilize Anti-CD4 to biosensors, flow CD4-his over. Monitor level of binding.
Recombinant Human CD4 Protein, CF

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Reason for Rating: Good binding with CD4-his to Anti-CD4. KD ~5nM

Verified Customer | Posted 02/05/2021

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