Recombinant Human CD30/TNFRSF8 Protein, CF

Name: Recombinant Human CD30/TNFRSF8 Protein, CF
Brand: R&D Systems
SKU: 6126-CD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 6126-CD

Product Datasheet / COA / SDS

rh CD30/TNFRSF8 (Catalog # 813-CD) is also available with a Fc and a His tag!

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Key Product Details

Source:	NS0
Accession #:	P28908.1
Structure / Form:	Non-covalently linked trimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human CD30/TNFRSF8 Protein, CF

Source	Mouse myeloma cell line, NS0-derived human CD30/TNFRSF8 protein Phe19-Lys379
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Phe19
Predicted Molecular Mass	38.4 kDa (monomer)
SDS-PAGE	65-85 kDa, reducing conditions
Activity	Measured by its ability to block CD30 Ligand-induced IL-6 secretion by HDLM human Hodgkin’s lymphoma cells. The ED₅₀ for this effect is 1-5 μg/mL in the presence of 50 ng/mL of Recombinant Human CD30 Ligand (Catalog # 1028-CL).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6126-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD30/TNFRSF8

CD30, also known as Ki-1 antigen and TNFRSF8, is a 120 kDa type I transmembrane glycoprotein belonging to the TNF receptor superfamily (1, 2). Mature human CD30 consists of a 361 amino acid (aa) extracellular domain (ECD) with six cysteine-rich repeats, a 28 aa transmembrane segment, and a 188 aa cytoplasmic domain (3). In contrast, mouse and rat CD30 lack 90 aa of the ECD and contain only three cysteine-rich repeats. Within common regions of the ECD, human CD30 shares 53% and 49% aa sequence identity with mouse and rat CD30, respectively. Alternate splicing of human CD30 generates an isoform that includes only the C‑terminal 132 aa of the cytoplasmic domain. CD30 is normally expressed on antigen-stimulated Th cells and B cells (4 ‑ 6). However, it is up‑regulated in Hodgkin’s disease (on Reed-Sternberg cells), other lymphomas, chronic inflammation, and autoimmunity (7). CD30 binds to CD30 Ligand/TNFSF8 which is expressed on activated Th cells, monocytes, granulocytes and medullary thymic epithelial cells (1, 5). CD30 signaling co‑stimulates antigen-induced Th0 and Th2 proliferation and cytokine secretion but favors a Th2-biased immune response (8). In the absence of antigenic stimulation, it can still induce T cell expression of IL‑13 (9). CD30 contributes to thymic negative selection by inducing the apoptotic cell death of CD4+CD8+ T cells (10, 11). In B cells, CD30 ligation promotes cellular proliferation and antibody production in addition to the expression of CXCR4, CCL3, and CCL5 (5, 12). An 85 ‑ 90 kDa soluble form of CD30 is shed from the cell surface by TACE-mediated cleavage (13, 14). Soluble CD30 retains the ability to bind CD30 Ligand and functions as an inhibitor of normal CD30 signaling (15).

Item	Value
References	Kennedy, M.K. et al. (2006) Immunology 118:143. Tarkowski, M. (2003) Curr. Opin. Hematol. 10:267. Durkop, H. et al. (1992) Cell 68:421. Hamann, D. et al. (1996) J. Immunol. 156:1387. Shanebeck, S.D. et al. (1995) Eur. J. Immunol. 25:2147. Gruss, H.-J. et al. (1994) Blood 83:2045. Oflzoglu E. et al. (2009) Adv. Exp. Med. Biol. 647:174. Del Prete, G. et al. (1995) J. Exp. Med. 182:1655. Harlin, H. et al. (2002) J. Immunol. 169:2451. Amakawa, R. et al. (1996) Cell 84:551. Chiarle, R. et al. (1999) J. Immunol. 163:194. Vinante, F. et al. (2002) Blood 99:52. Hansen, H.P. et al. (1995) Int. J. Cancer 63:750. Hansen, H.P. et al. (2000) J. Immunol. 165:6703. Hargreaves, P.G. and A. Al-Shamkhani (2002) Eur. J. Immunol. 32:163.
Entrez Gene IDs	943 (Human); 21941 (Mouse); 25069 (Rat)
Alternate Names	CD30, CD30 antigen, CD30KI-1, CD30L receptor, D1S166EKi-1, Ki-1 antigen, Lymphocyte activation antigen CD30, TNFRSF8, cytokine receptor CD30, tumor necrosis factor receptor superfamily member 8, tumor necrosis factor receptor superfamily, member 8

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Recombinant Human CD30/TNFRSF8 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human CD30/TNFRSF8 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: CD30/TNFRSF8

Citations for Recombinant Human CD30/TNFRSF8 Protein, CF

Product Documents for Recombinant Human CD30/TNFRSF8 Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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