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Recombinant Human CD3 gamma Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10550-CD

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10550-CD-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD3 gamma protein
Human CD3 gamma
(Gln23-Ser116)
Accession # P09693.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln23 inferred from enzymatic pyroglutamate treatment revealing Ser24

Predicted Molecular Mass

37 kDa

SDS-PAGE

42-53 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD3 gamma Fc Chimera (Catalog # 10550-CD) is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3E Fc Chimera that produces 50% of the optimal binding response is approximately 0.5-4 μg/mL.

Scientific Data Images for Recombinant Human CD3 gamma Fc Chimera Protein, CF

Recombinant Human CD3 gamma Fc Chimera Protein Binding Activity

Recombinant Human CD3 gamma Fc Chimera Protein Binding Activity

When Recombinant Human CD3 gamma Fc Chimera (Catalog # 10550-CD) is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3E Fc Chimera that produces 50% of the optimal binding response is approximately 0.5-4 μg/mL.

Recombinant Human CD3 gamma Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human CD3 gamma Fc Chimera Protein (Catalog # 10550-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 42-53 kDa and 80-110 kDa, respectively.

Formulation, Preparation and Storage

10550-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD3 gamma

T-cell surface glycoprotein CD3 gamma chain, also known as CD3G, is a 20 kDa single-pass type I membrane protein that is part of the TCR-CD3 complex. CD3 gamma forms a heterodimer with CD3 epsilon which associates with TCR beta, while CD3 delta/CD3 epsilon heterodimer associates with TCR alpha. CD3 gamma/CD3 epsilon/TCR beta joins CD3 delta/CD3 epsilon/TCR alpha and recruits a CD3 zeta homodimer to form the complete TCR-CD3 complex (1). CD3 chains contain one cytoplasmic ITAM domain which mediates intracellular signal transduction (2). The extracellular domain of CD3 gamma contains one disulfide bond and 2 N-linked glycosylation sites. The ECD of human CD3 gamma shares 60% and 62% sequence identity with mouse and rat CD3 gamma, respectively. The biologic roles of each CD3 chain and their relative contribution to the signals transduced through the TCR/CD3 complex and to downstream activation events are still uncertain, whether they may be specialized or redundant (3). Deficiencies in CD3 gamma can cause impairments in immune function, particularly in Treg cells which may contribute to autoimmune disorders (4).

References

  1. Kuhns, M. et al. (2006). Immunity 24:133.
  2. Hayes, S.M. et al. (2003). Immunol. Rev. 191:28.
  3. Pacheco-Castro, A. et al. (1998) J. Immunol. 161:3152.
  4. Rowe, J. et al. (2018). Blood. 131:2335.

Alternate Names

CD3g, IMD17, T3G

Entrez Gene IDs

917 (Human); 12502 (Mouse); 300678 (Rat); 102134381 (Cynomolgus Monkey)

Gene Symbol

CD3G

UniProt

Additional CD3 gamma Products

Product Documents for Recombinant Human CD3 gamma Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CD3 gamma Fc Chimera Protein, CF

For research use only

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