Recombinant Human CD25/IL-2R alpha Fc Chimera Protein, CF

Name: Recombinant Human CD25/IL-2R alpha Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1020-RL
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1020-RL

Citations (2)

(1)

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	Q5W007
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human CD25/IL-2R alpha Fc Chimera Protein, CF

Product Specifications for Recombinant Human CD25/IL-2R alpha Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human CD25/IL-2R alpha protein
class=TableText7 align="right">

Human CD25/IL-2 R alpha (Glu22-Cys213) Accession # Q5W007	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Glu22

Predicted Molecular Mass

48.3 kDa (monomer)

SDS-PAGE

65 kDa, reducing conditions

Activity

Measured by its ability to inhibit the IL-2-dependent proliferation of MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED₅₀ for this effect is 0.1-0.5 µg/mL in the presence of 30 ng/mL of recombinant human IL-2.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1020-RL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD25/IL-2R alpha

Human IL-2 receptor alpha (IL-2 R alpha ), also known as Tac antigen and CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL-2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed.

Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.

Item	Value
Long Name	Interleukin 2 Receptor alpha
Entrez Gene IDs	3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)
Alternate Names	CD25, CD25 antigen, IDDM10, IL-2 R alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL-2Ra, IL2-RA, IL2R, IL2R alpha, IL2RA, TAC antigen, TCGFR, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55

Citations for Recombinant Human CD25/IL-2R alpha Fc Chimera Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human CD25/IL-2R alpha Fc Chimera Protein, CF include:

Species: Human
Sample Types: Recombinant Proteins
Applications: ELISA Capture

K See et al. (2021), Antibody-guided design and identification of CD25-binding small antibody mimetics using mammalian cell surface display Scientific Reports, 11(1):22098.
PMID: 34764369
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Luissint AC et al. (2008), JAM-L-mediated leukocyte adhesion to endothelial cells is regulated in cis by alpha4beta1 integrin activation. J. Cell Biol., 183(6):1159-73.
PMID: 19064666

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Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 11/06/2019

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Product Documents for Recombinant Human CD25/IL-2R alpha Fc Chimera Protein, CF

Product Datasheets

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SDS

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