Source	Human embryonic kidney cell, HEK293-derived human CD200R1 protein Ala27-Leu266, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala27
Predicted Molecular Mass	28 kDa
SDS-PAGE	43-60 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinan Human CD200 R1 is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) that produces 50% of the optimal binding response is 2.5-15 ng/mL.

Recombinant Human CD200R1 His-tag Protein, CF Scientific Data Examples

	Recombinant Human CD200R1 His-tag Protein Binding Activity When Recombinant Human CD200 R1 (Catalog # 10053-CD) is coated at 2 µg/mL, 100 µL/well, Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) binds with an ED₅₀ of 2.5-15 ng/mL.
	Recombinant Human CD200R1 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Human CD200 R1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 43-60 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10053-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD200R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa transmembrane protein in the immunoglobulin superfamily and is important in the regulation of myeloid cell activity (1-3). The human CD200 R1 cDNA encodes a 325 amino acid (aa) precursor that includes a 28 aa signal sequence, a 215 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 61 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, human CD200 R1 shares 56% aa sequence identity with both mouse and rat CD200 R1. Alternate splicing of the human CD200 R1 mRNA generates four isoforms, two of which are truncated in the Ig-C2 domain and are likely secreted (4). In human, a separate CD200 RL gene encodes a protein that shares 81% ECD aa identity with CD200 R1 (5). In mouse, at least four genes for CD200 R1-like molecules have been described (5-7). CD200 R1 expression is restricted primarily to mast cells, basophils, macrophages, and dendritic cells (8-10), while its ligand, CD200, is widely distributed (11). Disruption of this receptor-ligand system by knockout of the CD200 gene in mice leads to increased macrophage number and activation and predisposition to autoimmune disorders (12). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (13). The capacity of CD200 R1-like molecules to interact with CD200 is controversial (6, 14). CD200 R1 propagates inhibitory signals despite lacking a cytoplasmic ITIM (immunoreceptor tyrosine-based inhibitory motif) (9, 10, 15, 16). CD200 R1-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (5, 7). CD200R1 signaling inhibits the expression of proinflammatory molecules including TNFs, IFNs, and inducible nitric oxide synthase in response to selected stimuli, which implicate that CD200/CD200R1 inhibitory signaling pathway plays a prominent role in limiting inflammation in a wide range of inflammatory diseases (17). Furthermore, the CD200/CD200R inhibitory signaling constitutes one of the most suitable endogenous immunoregulatory molecule candidate to restore the immune suppressive status of the CNS altered in chronic neuroinflammatory situations (18).

Item	Value
References	Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483. Barclay, A.N. et al. (2002) Trends Immunol. 23:285. Vieites J.M. et al. (2003) Gene. Jun. 5; 311:99. Wright, G.J. et al. (2003) J. Immunol. 171:3034. Hatherley, D. et al. (2005) J. Immunol. 175:2469. Voehringer, D. et al. (2004) J. Biol. Chem. 279:54117. Shiratori, I. et al. (2005) J. Immunol. 175:4441. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348. Fallarino, F. et al. (2004) J. Immunol. 173:3748. Wright, G.J. et al. (2001) Immunology 102:173. Hoek, R.M. et al. (2000) Science 290:1768. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688. Gorczynski, R. et al. (2004) J. Immunol. 172:7744. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191. Zhang, S. et al. (2004) J. Immunol. 173:6786. Vaine, C.A. et al. (2014) Adv Immunol.121:191. Hernangómez, M. et al. (2014) Curr Pharm Des. 20:4707.
Long Name	CD200 Receptor 1
Entrez Gene IDs	131450 (Human); 57781 (Mouse); 102139962 (Cynomolgus Monkey)
Alternate Names	CD200 R1, CD200 receptor 1, CD200R1, CD200RMOX2Rcell surface glycoprotein CD200 receptor 1, CRTR2, Cell surface glycoprotein OX2 receptor 1, HCRTR2, MOX2 receptor, MOX2R, OX2RCD200 cell surface glycoprotein receptor, cell surface glycoprotein receptor CD200

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Recombinant Human CD200R1 His-tag Protein, CF

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