Human embryonic kidney cell, HEK293-derived human CD200R1 protein Ala27-Leu266, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
43-60 kDa, reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinan Human CD200 R1 is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) that produces 50% of the optimal binding response is 2.5-15 ng/mL.
Recombinant Human CD200R1 His-tag Protein, CF Scientific Data Examples
Recombinant Human CD200R1 His-tag Protein Binding Activity
When Recombinant Human CD200 R1 (Catalog # 10053-CD) is coated at 2 µg/mL, 100 µL/well, Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) binds with an ED50 of 2.5-15 ng/mL.
Recombinant Human CD200R1 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Human CD200 R1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 43-60 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
CD200 R1, also known as OX-2 receptor, is a 90 kDa transmembrane protein in the immunoglobulin superfamily and is important in the regulation of myeloid cell activity (1-3). The human CD200 R1 cDNA encodes a 325 amino acid (aa) precursor that includes a 28 aa signal sequence, a 215 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 61 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, human CD200 R1 shares 56% aa sequence identity with both mouse and rat CD200 R1. Alternate splicing of the human CD200 R1 mRNA generates four isoforms, two of which are truncated in the Ig-C2 domain and are likely secreted (4). In human, a separate CD200 RL gene encodes a protein that shares 81% ECD aa identity with CD200 R1 (5). In mouse, at least four genes for CD200 R1-like molecules have been described (5-7). CD200 R1 expression is restricted primarily to mast cells, basophils, macrophages, and dendritic cells (8-10), while its ligand, CD200, is widely distributed (11). Disruption of this receptor-ligand system by knockout of the CD200 gene in mice leads to increased macrophage number and activation and predisposition to autoimmune disorders (12). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (13). The capacity of CD200 R1-like molecules to interact with CD200 is controversial (6, 14). CD200 R1 propagates inhibitory signals despite lacking a cytoplasmic ITIM (immunoreceptor tyrosine-based inhibitory motif) (9, 10, 15, 16). CD200 R1-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (5, 7). CD200R1 signaling inhibits the expression of proinflammatory molecules including TNFs, IFNs, and inducible nitric oxide synthase in response to selected stimuli, which implicate that CD200/CD200R1 inhibitory signaling pathway plays a prominent role in limiting inflammation in a wide range of inflammatory diseases (17). Furthermore, the CD200/CD200R inhibitory signaling constitutes one of the most suitable endogenous immunoregulatory molecule candidate to restore the immune suppressive status of the CNS altered in chronic neuroinflammatory situations (18).
Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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