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Recombinant Human CCL2/MCP-1 Protein Best Seller

Bio-Techne includes R&D Systems | Catalog # 279-MC

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Carrier Free
279-MC-001MG/CF
279-MC-010/CF

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With Carrier
279-MC-010
279-MC-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human CCL2/JE/MCP-1 protein
Gln24-Thr99

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln24

Predicted Molecular Mass

8.7 kDa

SDS-PAGE

10 kDa, reducing conditions

Activity

Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR2A.
The ED50 for this effect is 5-30 ng/mL.

Scientific Data Images for Recombinant Human CCL2/MCP-1 Protein

Recombinant Human CCL2/MCP-1 Protein Bioactivity

Recombinant Human CCL2/MCP-1 Protein Bioactivity

Recombinant Human CCL2/ MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A. The ED50 for this effect is 5-30 ng/mL.
Recombinant Human CCL2/MCP-1 Protein SDS-PAGE

Recombinant Human CCL2/MCP-1 Protein SDS-PAGE

1 μg/lane of Recombinant Human CCL2/MCP-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 10 kDa.
Recombinant Human CCL2/MCP-1 Protein Mass Spectrometry

Recombinant Human CCL2/MCP-1 Protein Mass Spectrometry

MALDI-TOF analysis of Recombinant Human CCL2/MCP-1. The major peak corresponds to the calculated molecular mass, 8679 Da. The minor peak at 8887 is a matrix-associated artifact of the MALDI-TOF.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 279-MC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 279-MC/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL2/JE/MCP-1

CCL2, also called monocyte chemotactic protein‑1 (MCP‑1) or JE, is a member of the C‑C or beta chemokine family that is best known as a chemotactic agent for mononuclear cells (1, 2). Human CCL2 cDNA encodes a 99 amino acid (aa) precursor protein with a 23 aa signal peptide and a 76 aa mature protein (2). Removal of the first 5 aa of the mature protein, including the N-terminal pyrrolidone carboxylic acid‑modified glutamine, occurs naturally by metalloproteinase cleavage and down-regulates activity but not receptor binding (3). CCL2 may form multiple bands from 8.7‑13.5 kDa on SDS‑PAGE due to non‑covalent dimerization and variable carbohydrate content (3). Mature human CCL2 shares 78‑79% aa identity with canine, porcine and equine CCL2, while mouse and rat express a form of CCL2 that is extended by 49 aa and shares only ~56% aa identity within the common region. Human CCL2 can, however, induce a response in murine cells (4). Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation, but monocytes and macrophages appear to be the major source (1, 2). In addition to its chemotactic activity, CCL2 induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils that express its receptor, CCR2 (2). Additionally, it promotes Th2 polarization in CD4+ T cells (5). CCL2‑mediated recruitment of monocytes to sites of inflammation is proposed to play a role in the pathology of atherosclerosis, multiple sclerosis and allergic asthma (6, 7).

References

  1. Yoshimura, T. et al. (1989) FEBS Lett. 244:487.
  2. Deshmane, S.L. et al. (2009) J. Interferon Cytokine Res. 29:313.
  3. Proost, P. et al.(1998) J. Immunol. 160:4034.
  4. Ju Lee, H. et al. (2015) J. Immunol. 194:3634.
  5. Luther, S.A. and J.G. Cyster (2001) Nat. Immunol. 2:102.
  6. Daly, C. et al. (2003) Microcirculation 10:247.
  7. Aukrust, P. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1909.

    Alternate Names

    GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF

    Entrez Gene IDs

    6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

    Gene Symbol

    CCL2

    UniProt

    Additional CCL2/JE/MCP-1 Products

    Product Documents for Recombinant Human CCL2/MCP-1 Protein

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Product Specific Notices for Recombinant Human CCL2/MCP-1 Protein

    For research use only

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