Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Recombinant Human Cathepsin S Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1183-CY

Catalog #
Availability
Size / Price
Qty
Loading...
1183-CY-010

Key Product Details

Source

NS0

Accession #

Structure / Form

Pro and mature forms

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Cathepsin S protein
Gln17-Ile331 (pro) & Ser109-Ile331 (mature), both with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln17 predicted & Ser109

Predicted Molecular Mass

39 kDa (Pro) and 28 kDa (mature)

SDS-PAGE

37 kDa and 26 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >300 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

1183-CY
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Cathepsin S

Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II‑associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1‑16), a pro region (residues 17‑114), and the mature enzyme (residues 115‑331) (3‑5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages and other phagocytic cells.

References

  1. Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104 - 1107, Academic Press, San Diego.
  2. Turk, V. et al. (2001) EMBO J. 20:4629.
  3. Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
  4. Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
  5. Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.

Alternate Names

CTSS

Entrez Gene IDs

1520 (Human)

Gene Symbol

CTSS

UniProt

Additional Cathepsin S Products

Product Documents for Recombinant Human Cathepsin S Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Cathepsin S Protein, CF

For research use only

Citations for Recombinant Human Cathepsin S Protein, CF (16)

Showing  1 - 10 of 16 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Cathepsin S Protein, CF include:

There are no citations that match your criteria.

Showing  1 - 10 of 16 publications Showing All

Customer Reviews for Recombinant Human Cathepsin S Protein, CF (1)

5 out of 5
1 customer rating
5 stars
100%
4 stars
0%
3 stars
0%
2 stars
0%
1 star
0%
Showing  1 - 1 of 1 review Showing All
Filter By:
  • Recombinant Human Cathepsin S Protein, CF
    Name: Balaji Mahender
    Application: Immunoassay Standard
    Verified Customer | Posted 02/05/2018

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All