Recombinant Human Cardiotrophin-1/CT-1 Protein

Name: Recombinant Human Cardiotrophin-1/CT-1 Protein
Brand: R&D Systems
SKU: 612-CD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 612-CD

Citations (8)

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Key Product Details

Source:	E. coli
Accession #:	Q16619
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Cardiotrophin-1/CT-1 Protein

Product Specifications for Recombinant Human Cardiotrophin-1/CT-1 Protein

Source	E. coli-derived human Cardiotrophin-1/CT-1 protein Ser2-Ala201
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser2
Predicted Molecular Mass	21 kDa
Activity	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.25‑1.25 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

612-CD

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCL containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

612-CD/CF

Carrier Free

Formulation	Supplied as a 0.2 μm filtered solution in Acetonitrile and TFA.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cardiotrophin-1/CT-1

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third alpha receptor subunit has been implicated in the receptor complex.

Item	Value
References	Pennica, D. et al. (1996) Cytokine and Growth Factor Reviews 7:81. Robledo, O. et al. (1997) J. Biol. Chem. 272:4855.
Entrez Gene IDs	1489 (Human); 13019 (Mouse)
Alternate Names	CT-1CT1, CTF1, Cardiotrophin-1, Cardiotrophin1, cardiophin 1, cardiotrophin 1

Citations for Recombinant Human Cardiotrophin-1/CT-1 Protein (8)

Showing 1 - 8 of 8 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Cardiotrophin-1/CT-1 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Lindkvist et al. (2022), Pleiotropic, Unique and Shared Responses Elicited by IL-6 Family Cytokines in Human Vascular Endothelial Cells International Journal of Molecular Sciences, 23(3).
PMID: 35163371
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

E Martínez-M et al. (2017), Differential Proteomics Identifies Reticulocalbin-3 as a Novel Negative Mediator of Collagen Production in Human Cardiac Fibroblasts Sci Rep, 7(1):12192.
PMID: 28939891
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Emdad L et al. (2012), Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes. Stem Cells Dev., 21(3):404-10.
PMID: 21631388
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Larsen JV et al. (2010), Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer. Mol. Cell. Biol., 17(0):4175.
PMID: 20584990
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Duluc D et al. (2009), Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages. Int. J. Cancer, 125(2):367-73.
PMID: 19378341
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Fritzenwanger M et al. (2007), Cardiotrophin-1 induces interleukin-6 synthesis in human monocytes. Cytokine, 38(3):137-44.
PMID: 17637508
Species: N/A
Sample Types: N/A
Applications: Western Blot

Gard AL et al. (2004), Cardiotrophin-1 in choroid plexus and the cerebrospinal fluid circulatory system. Neuroscience, 127(1):43-52.
PMID: 15219667
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Freed DH et al. (2003), Induction of protein synthesis in cardiac fibroblasts by cardiotrophin-1: integration of multiple signaling pathways. Cardiovasc. Res., 60(2):365-75.
PMID: 14613866

Recombinant Human Cardiotrophin-1/CT-1 Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human Cardiotrophin-1/CT-1 Protein

Formulation, Preparation and Storage

Carrier Free

612-CD

Carrier

612-CD/CF

Carrier Free

Reconstitution Calculator

Background: Cardiotrophin-1/CT-1

Citations for Recombinant Human Cardiotrophin-1/CT-1 Protein (8)

Product Documents for Recombinant Human Cardiotrophin-1/CT-1 Protein

Product Datasheets

COA

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