Recombinant Human Carbonic Anhydrase XIV Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2195-CA
Key Product Details
- R&D Systems NS0-derived Recombinant Human Carbonic Anhydrase XIV Protein (2195-CA)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Carbonic Anhydrase XIV/CA14 protein
Gly19-Met290, with a C-terminal 10-His tag
Gly19-Met290, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly19
Predicted Molecular Mass
32 kDa
SDS-PAGE
43-50 kDa, reducing conditions
Activity
Measured by its esterase activity.
The specific activity is >100 pmol/min/µg, as measured under the described conditions.
The specific activity is >100 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2195-CA
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Carbonic Anhydrase XIV/CA14
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as anutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA14 is a type I membrane enzyme highly expressed in all parts of the central nervous system with lower expression in adult liver, heart, small intestine, colon, kidney, urinary bladder, and skeletal muscle (2).
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Fujikawa-Adachi, K. et al. (1999) Genomics 61:74.
Alternate Names
CA14
Gene Symbol
CA14
UniProt
Additional Carbonic Anhydrase XIV/CA14 Products
Product Documents for Recombinant Human Carbonic Anhydrase XIV Protein, CF
Product Specific Notices for Recombinant Human Carbonic Anhydrase XIV Protein, CF
For research use only
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