Product Specifications for Recombinant Human Carbonic Anhydrase VI Protein, CF
Mouse myeloma cell line, NS0-derived human Carbonic Anhydrase VI protein Gln18-Asn308, with a C-terminal 10-His tag
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
No results obtained: Gln18 predicted
Predicted Molecular Mass
42 kDa, reducing conditions
Measured by its esterase activity. The specific activity is >3 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris, NaCl, Brij and Glycerol.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Do not freeze.
3 months from date of receipt, 2 to 8 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
Background: Carbonic Anhydrase VI
Carbonic Anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase VI, also known as gustin and salivary Carbonic Anhydrase, is a zinc-metalloprotein that constitutes about 3% of human parotid saliva protein (2, 3). It was decreased in patients with loss of taste and pathological changes in taste buds (4). It is also an elementary component of milk. It plays an important role in normal growth and development of the infant alimentary tract (5).
Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Murakami, H. and Sly, W. S. (1987) J. Biol. Chem. 262:1382.
Thatcher, B. J. et al. (1998) Biochem. Biophys. Res. Commun. 250:635.
Hankin, R. I. et al. (1999) Am. J. Med. Sci. 318:380.
Karhumaa, P. et al. (2001) Proc. Natl. Acad. Sci. USA. 98:11604.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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