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Recombinant Human Carbonic Anhydrase VB Protein, CF

Bio-Techne includes R&D Systems | Catalog # 3176-CA

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3176-CA-010

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

E. coli-derived human Carbonic Anhydrase VB/CA5B protein
Cys34-Pro317, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys34 & Ser35

Predicted Molecular Mass

34 kDa

SDS-PAGE

34 kDa, reducing conditions

Activity

Measured by its esterase activity.
The specific activity is >150 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

3176-CA
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Carbonic Anhydrase VB/CA5B

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20 - 25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase VB encoded by the CA5B gene is a mitochondrial protein. In comparison with another mitochondrial CA (CA5A), CA5B has different tissue distribution and chromosomal location (2, 3). The amino acid sequence of human CA5B (residues 34 to 317) is 94%, 93%, 92%, and 74% identical to that of canine, mouse, bovine/rat, and chicken.

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Fujikawa-Adachi, K. et al. (1999) J. Biol. Chem. 274:21228.
  3. Shah, G.N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1677.

Alternate Names

CA5B, CAVB

Entrez Gene IDs

11238 (Human); 302669 (Rat)

Gene Symbol

CA5B

UniProt

Additional Carbonic Anhydrase VB/CA5B Products

Product Documents for Recombinant Human Carbonic Anhydrase VB Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Carbonic Anhydrase VB Protein, CF

For research use only