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Recombinant Human Cadherin-13 (CAD-13) Protein, CF

Bio-Techne includes R&D Systems | Catalog # 3264-CA

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Product Specifications


Mouse myeloma cell line, NS0-derived human Cadherin-13 protein
Glu23-Ala692, with a C-terminal 6-His tag


>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

74.5 kDa


100-115 kDa, reducing conditions


Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells.

When 5 x 104 cells/well are added to rhCadherin-13 coated plates, cell adhesion is inhibited in a dose dependent manner after 2 hours at 37 °C. The ED50 for this effect is 2-8 μg/mL.

Formulation, Preparation and Storage

Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cadherin-13

Cadherin-13, also known as T-Cadherin and H‑Cadherin, is a GPI-anchored protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Following removal of its C‑terminal propeptide, Cadherin-13 is expressed on the cell surface both with and without its N-terminal propeptide in species of approximately 120 kDa and 100 kDa, respectively (3). Human Cadherin-13 contains five tandem Cadherin repeats and shares 95% aa sequence identity with mouse and rat Cadherin-13 (4). It is most highly expressed in the circulatory and nervous systems, particularly on cardiac myocytes, vascular endothelial and smooth muscle cells, and neurons and astrocytes in the brain (3, 5 - 9). Cadherin-13 exerts a negative influence on neurite extension, and it is down‑regulated in neurons upon NGF stimulation (10, 11). Homotypic Cadherin-13 interactions promote intercellular adhesion which can be inhibited by its binding to LDL (12). Cadherin-13 also mediates the cardioprotective effects of Adiponectin by directly binding Adiponectin (selectively the hexameric and HMW forms) and trapping it on vascular endothelial cells and cardiomyocytes (6 ‑ 8). The role of Cadherin-13 in the vasculature is complex. When expressed on vascular endothelial cells it promotes angiogenesis, but when expressed on stromal cells it inhibits neovascularization (13, 14). Its down‑regulation on breast carcinoma cells and up‑regulation on the vasculature of various tumors limits tumor cell proliferation and angiogenesis but also enhances tumor progression (4, 8, 15).


  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Philippova, M. et al. (2009) Cell. Signal. 21:1035.
  3. Stambolsky, D.V. et al. (1999) Biochim. Biophys. Acta 1416:155.
  4. Lee, S.W. (1996) Nat. Med. 2:776.
  5. Doyle, D.D. et al. (1998) J. Biol. Chem. 273:6937.
  6. Denzel, M.S. et al. (2010) J. Clin. Invest. 120:4342.
  7. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
  8. Hebbard, L.W. et al. (2008) Cancer Res. 68:1407.
  9. Takeuchi, T. et al. (2000) J. Neurochem. 74:1489.
  10. Fredette, B.J. et al. (1996) Development 122:3163.
  11. Bai, S. et al. (2007) J. Biol. Chem. 282:27171.
  12. Resink, T.J. et al. (1999) FEBS Lett. 463:29.
  13. Philippova, M. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26:2222.
  14. Rubina, K. et al. (2007) Angiogenesis 10:183.
  15. Wyder, L. et al. (2000) Cancer Res. 60:4682.

Alternate Names

Cadherin13, CDH13, H-Cadherin, T-Cadherin

Entrez Gene IDs

1012 (Human); 12554 (Mouse); 192248 (Rat)

Gene Symbol



Additional Cadherin-13 Products

Product Documents for Recombinant Human Cadherin-13 (CAD-13) Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Cadherin-13 (CAD-13) Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.