Recombinant Human Cadherin-11 (CAD-11) Fc Chimera, CF

Name: Recombinant Human Cadherin-11 (CAD-11) Fc Chimera, CF
Brand: R&D Systems
SKU: 1790-CA

Catalog # 1790-CA | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	AAA35622
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Technical Data
Citations (2) / Reviews

Product Specifications for Recombinant Human Cadherin-11 (CAD-11) Fc Chimera, CF

Source	Mouse myeloma cell line, NS0-derived human Cadherin-11 protein
Purity	>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.1 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Phe23 (pro) & Gly54 (mature)
Predicted Molecular Mass	93 kDa (pro), 90 kDa (mature)
SDS-PAGE	93 kDa (pro), 90 kDa (mature)
Activity	Measured by the ability of the immobilized protein to support the adhesion of MDA‑MB‑231 human breast cancer cells. When 7.5 x 10⁴ cells/well are added to Recombinant Human Cadherin-11 Fc Chimera coated plates (20 µg/mL with 100 µL/well), ≥50% of cells will adhere after 1 hour at 37 °C.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1790-CA

Formulation	Lyophilized from a 0.2 μm filtered solution in MES and NaCl with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Cadherin-11

Cadherin-11, also known as OB-Cadherin, is a 120 kDa member of the classical Cadherin family of calcium-dependent homophilic adhesion proteins. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1). Cadherin-11 is expressed in embryonic mesodermal tissues and contributes to the morphogenesis of the nervous and skeletal systems (2-4). It is expressed on osteoblasts in the adult where it promotes the differentiation of both osteoblasts and chondrocytes (5). Cadherin-11 is up‑regulated on breast cancer and prostate cancer cells which preferentially metastasize to bone (6, 7). It facilitates this metastasis via homophilic adhesion to bone marrow stroma and osteoblast-expressed Cadherin-11 (6‑8). In the synovium, Cadherin‑11 supports adhesion between synoviocytes but promotes cell invasion in synovitis and rheumatoid arthritis (9, 10). Its up‑regulation in the vasculature following injury contributes to intimal hyperplasia by inducing smooth muscle cell migration and proliferation (11). In the nervous system, Cadherin-11 interacts with FGF R1 to promote neurite extension from spinal cord explants (12). Mature human Cadherin‑11 consists of a 564 amino acid (aa) extracellular domain (ECD) with five tandem Cadherin repeats, a 23 aa transmembrane segment, and a 156 aa cytoplasmic domain (13, 14). Within the ECD, human Cadherin-11 shares 97% and 98% aa sequence identity with mouse and rat Cadherin‑11, respectively. An 80 kDa portion of the Cadherin‑11 ECD can be shed by proteolytic cleavage, and this fragment competes with the full length molecule for cell adhesion (3, 15). Alternate splicing of human Cadherin-11 generates an 85 kDa isoform with substituted transmembrane and cytoplasmic regions (14, 15).

Item	Value
Entrez Gene IDs	1009 (Human); 12552 (Mouse)
Alternate Names	CAD11OSF-4, CDH11, CDHOB, Cadherin-11, Cadherin11, OB, OB-Cadherin, Osteoblast cadherin, cadherin 11, type 2, OB-cadherin (osteoblast)

Citations for Recombinant Human Cadherin-11 (CAD-11) Fc Chimera, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Cadherin-11 (CAD-11) Fc Chimera, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Binding Assay

Chu K et al. (2008-08-01), Cadherin-11 promotes the metastasis of prostate cancer cells to bone. Mol. Cancer Res., 2008-06(8):1259-67.
PMID: 18708358
Species: Rat
Sample Types: Whole Cells
Applications: Binding Assay

Pittet P et al. (2008-02-26), Fibrogenic fibroblasts increase intercellular adhesion strength by reinforcing individual OB-cadherin bonds. J. Cell. Sci., 2008-0121(0):877-86.
PMID: 18303045

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Product Documents for Recombinant Human Cadherin-11 (CAD-11) Fc Chimera, CF

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