Recombinant Human C1qR1/CD93 (24-580) His Avi Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11656

His-tag Avi-tag
R&D Systems, part of Bio-Techne
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AVI11656-050
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Key Product Details

Source

HEK293

Accession #

Q9NPY3.3

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all C1qR1/CD93 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human C1qR1/CD93 protein
Ala24-Lys580, with a C-terminal 6-His tag and Avi-tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala24

Predicted Molecular Mass

61 kDa

SDS-PAGE

82-105 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human C1qR1/CD93 (24-580) His-tag Avi-tag binds to Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) Protein (Catalog # 1334-B7) with an ED50 of 1.50-15.0 μg/mL.

Scientific Data Images for Recombinant Human C1qR1/CD93 (24-580) His Avi Protein, CF

Biotinylated Recombinant Human C1qR1/CD93 (24-580) His-tag Avi-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human C1qR1/CD93 (24-580) His-tag Avi-tag Protein (Catalog # AVI11656) binds to Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) Protein (1334-B7) with an ED50 of 1.50-15.0 μg/mL.

Biotinylated Recombinant Human C1qR1/CD93 (24-580) His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human C1qR1/CD93 (24-580) His-tag Avi-tag Protein (Catalog # AVI11656) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 82-105 kDa.

Formulation, Preparation and Storage

AVI11656
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C1qR1/CD93

C1qR1, also known as CD93 and C1qRp, is an approximately 125 kDa type-1 transmembrane glycoprotein that is involved in various aspects of inflammatory reactions (1). Mature human CD93 consists of a 557 amino acid (aa) extracellular domain (ECD) containing C‑type lectin and EGF‑like domains, followed by a 21 aa transmembrane segment and a 51 aa cytoplasmic domain (2, 3). Within the ECD, human CD93 shares 65% aa sequence identity with mouse and rat CD93. CD93 is expressed by vascular endothelial cells (5) and by a variety of hematopoietic cells (3‑9). Various sized fragments of soluble CD93 (50‑75 kDa) can be shed from monocytes, neutrophils, and vascular endothelial cells following inflammatory stimulation, leaving a residual stub in the membrane (11‑13). Cross‑linking of cell surface CD93 enhances phagocytosis by monocytes and enhances the uptake of apoptotic cells in vivo (10, 15). Soluble CD93 promotes the differentiation of monocytes to macrophages, phagocytosis of apoptotic cells, and inflammatory responsiveness to multiple TLR ligands (12, 14). CD93 plays a role in cardiovascular disease progression and modulates angiogenesis, inflammation and tumor growth and its interaction with insulin-like growth factor binding protein 7 (IGFBP7) contributes to abnormal tumor vasculature (16-17). Our Avi-tag Biotinylated Human C1qR1/CD93 (24-580) features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Greenlee-Wacker, M.C. et al. (2012) Curr. Drug Targets 13:411.
  2. Nepomuceno, R.R. et al. (1997) Immunity 6:119.
  3. Steinberger, P. et al. (2002) J. Leukoc. Biol. 71:133.
  4. Nepomuceno, R.R. and A.J. Tenner (1998) J. Immunol. 160:1929.
  5. McGreal, E.P. et al. (2002) J. Immunol. 168:5222.
  6. Lovik, G. et al. (2001) Scand. J. Immunol. 53:410.
  7. Danet, G.H. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10441.
  8. Ikewaki, N. et al. (2010) J. Clin. Immunol. 30:723.
  9. Chevrier, S. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3895.
  10. Norsworthy, P.J. et al. (2004) J. Immunol. 172:3406.
  11. Bohlson, S.S. et al. (2005) J. Immunol. 175:1239.
  12. Greenlee, M.C. et al. (2009) Inflamm. Res. 58:909.
  13. Greenlee-Wacker, M.C. et al. (2011) J. Immunol. 187:3353.
  14. Jeon, J.W. et al. (2010) J. Immunol. 185:4921.
  15. Nepomuceno, R.R. et al. (1999) J. Immunol. 162:3583.
  16. Tossetta, G. et al. (2023) Cells 12:1778.
  17. Sun, Y. et al. (2021) Sci. Transl. Med. 13:eabc8922.

Long Name

Complement Component C1q Receptor

Alternate Names

AA4 Antigen, C1q R1, C1q Rp, C1qRp, CD93, Collectin Receptor, Ly68

Entrez Gene IDs

22918 (Human); 17064 (Mouse); 84398 (Rat)

Gene Symbol

CD93

UniProt

Q9NPY3.3

Additional C1qR1/CD93 Products

Product Documents for Recombinant Human C1qR1/CD93 (24-580) His Avi Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human C1qR1/CD93 (24-580) His Avi Protein, CF

For research use only

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