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Recombinant Human BTN3A3 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1350-BT

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1350-BT-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human BTN3A3 protein
Gln30-Trp248, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln30

Predicted Molecular Mass

24 kDa

SDS-PAGE

28-37 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect is 1-6 μg/mL.

Scientific Data Images for Recombinant Human BTN3A3 His-tag Protein, CF

Recombinant Human BTN3A3 His-tag Protein Bioactivity

Recombinant Human BTN3A3 His-tag Protein Bioactivity

Recombinant Human BTN3A3 (Catalog # 1350-BT) inhibits anti-CD3 antibody induced IFN-gamma secretion by human T cells. The ED50 for this effect is 1-6 μg/mL.
Recombinant Human BTN3A3 His-tag Protein SDS-PAGE

Recombinant Human BTN3A3 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human BTN3A3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 28 -37 kDa.

Formulation, Preparation and Storage

1350-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTN3A3

BTN3A3 (Butyrophylin subfamily 3 member A3), is a type 1 transmembrane glycoprotein belonging to the Ig superfamily. BTN molecules have three subfamilies, BTN1-3 and are composed of two Ig domains, a single transmembrane domain, and a large C-terminal domain located in the cell cytoplasm (1, 2). Mature human BTN3A3 consists of a 219 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 315 aa cytoplasmic domain. Within the ECD, human BTN3A3 shares 45% and 41% aa sequence identity with mouse and rat BTN3A3, respectively. The BTN3A subfamily of butyrophilins (BTN3A1, BTN3A2 and BTN3A3) are expressed by most human immune cell subsets, including T cells, B cells, monocytes, dendritic cells and natural killer (NK) cells (2, 3). In in vitro studies, the BTN3A-specific monoclonal antibody bound to BTN3A directly on the T cell surface, driving the phosphorylation of the subfamily member BTN3A3, and initiating an inhibitory signaling cascade that resulted in decreased T cell proliferation and cytokine production (2). Single nucleotide polymorphisms (SNPs) in BTN3A3 was reported to be associated with increased susceptibility to ovarian cancer, supporting the notion that butyrophilins might contribute to immune evasion in oncology settings (4).

References

  1. Rhodes, D.A. et al. (2015) J. Immunol. 194:2390.
  2. Yamashiro, H. et al. (2010) J. Leukoc. Biol. 88:757.
  3. Messal, N. et al. (2011) Eur. J. Immunol. 41:3443.
  4. Peedicayil, A. et al. (2010) PLoS ONE 5:e8884.

Long Name

Butyrophilin, Subfamily 3, Member A3

Alternate Names

BTF3, BTN3.3

Entrez Gene IDs

10384 (Human)

Gene Symbol

BTN3A3

UniProt

Additional BTN3A3 Products

Product Documents for Recombinant Human BTN3A3 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human BTN3A3 His-tag Protein, CF

For research use only

Reconstitution Calculator

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