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Recombinant Human BMP-8a Protein

Catalog # 1073-BP | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
1073-BP-010/CF

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With Carrier
1073-BP-010

Key Product Details

Source

E. coli

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

E. coli-derived human BMP-8a protein
Ala264-His402 & Val265-His402

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala264 & Val265

Predicted Molecular Mass

15.8 kDa (monomer)

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human BMP‑8a is present at 1 μg/mL, the concentration of recombinant human BMPR‑1A Fc Chimera that produces 50% of the optimal binding response is approximately 1.5‑6 μg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1073-BP
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 20 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1073-BP/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-8a

BMP-8, also known as osteogenic protein 2 (OP-2), was first isolated from a hippocampal library in a screen to identify relatives of BMP-7 (OP-1) (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. They have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs (2). BMPs play roles in regulating growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal, epithelial, hematopoetic, and neuronal cells.

There exist two highly related and closely linked genes, designated BMP-8a and -8b in mice and humans. For humans, the protein products of these two genes share 98% amino acid (aa) sequence identity in their pro- and mature regions. However in the mouse, the two proteins share 89% and 76% aa sequence homology in their pro- and mature regions, respectively (3). Mature human BMP-8a shares 91% and 70% aa sequence identity with mouse BMP-8a and -8b, respectively. Human BMP‑8a is synthesized as a large precursor protein that is cleaved at a dibasic cleavage site (RTPR) between aa residues 263 and 264 to release a 139 aa carboxy‑terminal domain. Expression patterns of the BMP-8 genes indicate that they regulate aspects of cell proliferation and/or differentiation during spermatogenesis and formation of the placenta (3). BMP-8 is also highly expressed in osteosarcomas (4).

References

  1. Ozkaynak, E. et al. (1992) J. Biol. Chem. 267:25220.
  2. Canalis, E. et al. (2003) Endocrine Rev. 24:218.
  3. Zhao, G-Q. et al. (1996) Mech. Dev. 57:159.
  4. Sulzbacher, I. et al. (2002) J. Clin. Pathol. 55:381.

Long Name

Bone Morphogenetic Protein 8a

Alternate Names

Op2, Osteogenic Protein 2

Entrez Gene IDs

353500 (Human); 12163 (Mouse)

Gene Symbol

BMP8A

UniProt

Product Documents for Recombinant Human BMP-8a Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human BMP-8a Protein

For research use only

Citations for Recombinant Human BMP-8a Protein (9)

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Customer Reviews for Recombinant Human BMP-8a Protein (2)

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  • Recombinant Human BMP-8a Protein
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    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 02/18/2021
    Recombinant Human BMP-8a Protein 1073-BP
  • Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 03/19/2019

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FAQs for Recombinant Human BMP-8a Protein

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  • Q: Is catalog #1073-BP recombinant human BMP-8a or BMP-8b?

    A: R&D Systems recombinant human BMP-8a was originally sold with the name BMP-8b. When the product was first introduced, its sequence matched the sequence definition for BMP-8b. With the sequencing of the human genome, there has been revision in accessions numbers which has led to the designation of this protein as BMP-8a. Although there has been no change in this product, the name was updated for consistency with the sequence definition. The product description was updated to recombinant human BMP-8a in 2010 so publications prior to this time may refer to this product as BMP-8b.

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