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Recombinant Human BMP-8a (CHO-expressed) Protein, CF

Catalog # 1073-BPC | R&D Systems, Inc. a Bio-Techne Brand
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1073-BPC-025

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer by SDS-PAGE under non-reducing conditions

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human BMP-8a protein
Ala264-His402

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala264

Predicted Molecular Mass

15.7 kDa (monomer)

SDS-PAGE

19 kDa, reducing conditions

Activity

Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. Miyazaki, H. et al. (2004) Oncogene 23:9326.
The ED50 for this effect is 0.5-5 μg/mL.

Formulation, Preparation and Storage

1073-BPC
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution
Reconstitute at 250 μg/mL in 4 mM HCl.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: BMP-8a

BMP-8, also known as osteogenic protein 2 (OP-2), was first isolated from a hippocampal library in a screen to identify relatives of BMP-7/OP-1 (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta) superfamily. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis. Cellular responses to BMPs are mediated by hetero‑oligomeric complexes of type I and type II serine/threonine kinase receptors (2 - 4). BMP‑8a and BMP-8b, produced from separate genes, share 98% aa sequence identity in human but only 74% in mouse within the mature regions. Human BMP‑8a is synthesized as a 402 aa precursor protein that is cleaved between Arg263 and Ala264 to release the C-terminal mature protein. Mature human BMP‑8a shares 90% and 68% aa sequence identity with mouse mature BMP‑8a and -8b, respectively. BMP‑8a is expressed during pregnancy in the deciduum and trophoblast cells, by inner root sheath cells of developing hair follicles, and by the epididymis and spermatids (5 - 7). In the mouse it cooperates with BMP-7 in the maintenance of spermatogenesis but is not required for the initiation of spermatogenesis (7, 8). BMP‑8b, in contrast, is required for both the initiation and maintenance of spermatogenesis (9). BMP‑8a is induced during osteoblast differentiation at the onset of mineralization and during the osteogenic phase of bone repair in osteoblasts and osteocytes (10 - 12). BMP‑8a/b is also highly expressed in osteosarcomas (13).

References

  1. Ozkaynak, E. et al. (1992) J. Biol. Chem. 267:25220.
  2. Chen, D. et al. (2004) Growth Factors 22:233.
  3. Bragdon, B. et al. (2010) Cell Signal. Oct 16 epub.
  4. Singh, A. and R.J. Morris (2010) Cytokine Growth Factor Rev. 21:299.
  5. Zhao, G.-Q. and B.L. Hogan (1996) Mech. Dev. 57:159.
  6. Ying, Y. and G.-Q. Zhao (2000) Biol. Reprod. 63:1781.
  7. Zhao, G.-Q. et al. (1998) Development 125:1103.
  8. Zhao, G.-Q. et al. (2001) Dev. Biol. 240:212.
  9. Zhao, G.-Q. et al. (1998) Genes Dev. 10:1657.
  10. van der Horst, G. et al. (2002) Bone 31:661.
  11. Cho, T.-J. et al. (2002) J. Bone Miner. Res. 17:513.
  12. Paic, F. et al. (2009) Bone 45:682.
  13. Sulzbacher, I. et al. (2002) J. Clin. Pathol. 55:381.

Long Name

Bone Morphogenetic Protein 8a

Alternate Names

Op2, Osteogenic Protein 2

Entrez Gene IDs

353500 (Human); 12163 (Mouse)

Gene Symbol

BMP8A

UniProt

Product Documents for Recombinant Human BMP-8a (CHO-expressed) Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human BMP-8a (CHO-expressed) Protein, CF

For research use only

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