What is the half-life of a recombinant or natural protein in culture at 37 °C?

Name: Recombinant Human BMP-3 Protein, CF
Brand: R&D Systems
SKU: 113-BP/CF

R&D Systems has not determined the half-life of recombinant or natural proteins in culture. For cell-based bioassays, the incubation period of the protein with the cells typically ranges from 48 to 96 hours. Assay conditions for each protein will need to be optimized by each researcher.

Recombinant Human BMP-3 Protein, CF

Catalog # 113-BP/CF

Citations (4)

Product Datasheet / COA / SDS

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Key Product Details

Source:	E. coli
Accession #:	P12645.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human BMP-3 Protein, CF

Product Specifications

Source	E. coli-derived human BMP-3 protein Gln363-Arg472, with an N-terminal Met
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	12.5 kDa (monomer)
Activity	Measured by its ability to inhibit BMP-2-induced activity in MC3T3‑E1 mouse preosteoblast cells. Daluiski, A. et al. (2001) Nature Genetics 27:84. 30 µg/mL of hBMP-3 will antagonize hBMP-2 (0.25 µg/mL) induction of alkaline phosphatase in MC3T3E1 cells by >50%.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

113-BP/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 1 mg/mL in sterile 35% Acetonitrile and 0.1% TFA.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-3

BMP-3, also known as osteogenin, the most abundant BMP in adult bone, is one of at least 15 structurally and functionally related BMPs, which are members of the TGF- beta superfamily (1 - 3). BMPs were originally identified as protein regulators of cartilage and bone formation. They have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs also regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types. Similar to most other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human and rat BMP-3 are 98% identical. BMP-3 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. Biologically active BMP-3 is a disulfide-linked homodimer of the carboxy-terminal 110 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond (4). The role of BMP-3 in bone is contradictory since, unlike osteogenin purified from bone, recombinant BMP-3 has not shown osteogenic function (5). Several studies indicate that BMP-3 is an inhibitor of osteogenic BMPs. BMP-3 dorsalizes Xenopus embryos, the opposite effect of BMP-2 or 4, which cause ventralization. BMP-3 inhibits alkaline phosphatase production and induction of osteoblastic target genes in undifferentiated mesenchymal and osteogenic cell lines that have been treated with BMP-2. BMP-3 also induces the expression of TGF-beta /activin responsive genes, but not BMP-responsive genes. Since the inhibitory effect is not due to direct competition with osteogenic BMPs, it has been suggested that BMP-3 activates signaling through an activin pathway, resulting in antagonism of osteogenesis induced by other BMPs.

Item	Value
References	Chen, D. et al. (2004) Growth Factors 22:233. Hino, J. et al. (2004) Front. Biosci. 9:1520. Bahamonde, M.E. and K.M. Lyons (2001) J. Bone and Joint Surgery 83-A (suppl 1):S156. Wozney, J.M. et al. (1998) Science 242:1528. Daluiski, A. et al. (2001) Nature Genetics 27:84.
Long Name	Bone Morphogenetic Protein 3
Entrez Gene IDs	651 (Human); 110075 (Mouse); 25667 (Rat)
Alternate Names	BMP-3, BMP-3A, BMP3, BMP3A, Bone morphogenetic protein 3A, Osteogenin, bone morphogenetic protein 3, bone morphogenetic protein 3 (osteogenic), bone morphogenetic protein-3

Citations for Recombinant Human BMP-3 Protein, CF (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human BMP-3 Protein, CF include:

Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Castonguay R et al. (2011), Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 286(34):30034-46.
PMID: 21737454
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Stewart A et al. (2010), BMP-3 promotes mesenchymal stem cell proliferation through the TGF-beta/activin signaling pathway. J. Cell. Physiol., 223(3):658-66.
PMID: 20143330
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Tseng YH et al. (2008), New role of bone morphogenetic protein 7 in brown adipogenesis and energy expenditure. Nature, 454(7207):1000-4.
PMID: 18719589
Species: Xenopus
Sample Types: In Vivo
Applications: In Vivo

Mogi K et al. (2003), Xenopus neurula left-right asymmetry is respeficied by microinjecting TGF-beta5 protein. Int. J. Dev. Biol., 47(1):15-29.
PMID: 12653248

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FAQs

What is the half-life of a recombinant or natural protein in culture at 37 °C?

R&D Systems has not determined the half-life of recombinant or natural proteins in culture. For cell-based bioassays, the incubation period of the protein with the cells typically ranges from 48 to 96 hours. Assay conditions for each protein will need to be optimized by each researcher.