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Recombinant Human Betacellulin Protein, Animal-Free

Bio-Techne includes R&D Systems | Catalog # BT-BTC-AFL

Intended for preclinical researchers who may transition to GMP Betacellulin for their clinical work
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BT-BTC-AFL-01M
BT-BTC-AFL-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human Betacellulin/BTC protein
Asp32-Gln118, with an N-terminal Met
Produced using non-animal reagents in an animal-free laboratory.

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met-Asp32

Predicted Molecular Mass

9.9 kDa

SDS-PAGE

12-15 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 0.100-1.50 ng/mL.

Scientific Data Images for Recombinant Human Betacellulin Protein, Animal-Free

Recombinant Human Betacellulin Protein, Animal-Free SDS-PAGE.

2 μg/lane of Animal-Free™ Recombinant Human Betacellulin Protein (Catalog # BT-BTC-AFL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 12-15 kDa.

Recombinant Human Betacellulin Protein, Animal-Free, Bioactivity.

Animal-FreeTM Recombinant Human Betacellulin (Catalog # BT-BTC-AFL) as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.100-1.50 ng/mL. Two independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of Animal-Free Betacellulin.

Equivalent Bioactivity of GMP, Animal-Free, and RUO grades of Recombinant Human Betacellulin/BTC.

Equivalent bioactivity of GMP (BT-BTC-GMP), Animal-Free (Catalog # BT-BTC-AFL) and RUO (BT-BTC) grades of Recombinant Human Betacellulin/BTC as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells (orange, green, red, respectively).

Formulation, Preparation and Storage

BT-BTC-AFL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100-500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Betacellulin/BTC

Betacellulin (BTC) is a new member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGF-alpha, amphiregulin, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF-7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells.

Alternate Names

BTC

Entrez Gene IDs

685 (Human); 12223 (Mouse)

Gene Symbol

BTC

UniProt

Additional Betacellulin/BTC Products

Product Documents for Recombinant Human Betacellulin Protein, Animal-Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.  

 Production

  • All molecular biology procedures use animal-free media and dedicated labware.
  • Dedicated fermentors are utilized in committed animal-free areas.

Purification

  • Protein purification columns are animal-free.
  • Bulk proteins are filtered using animal-free filters.
  • Purified proteins are stored in animal-free containers in a dedicated cold storage room.

    Quality Assurance

    • Low Endotoxin Level.
    • No impairment of biological activity.
    • High quality product obtained under stringent conditions.
    • For ex vivo research or bioproduction, additional documentation can be provided.

    Please read our complete Animal-Free Statement

    Product Specific Notices for Recombinant Human Betacellulin Protein, Animal-Free

    For research use only

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