Recombinant Human beta IG-H3 Protein, CF

Name: Recombinant Human beta IG-H3 Protein, CF
Brand: R&D Systems
SKU: 3409-BG

Catalog # 3409-BG | R&D Systems, Inc. a Bio-Techne Brand

Citations (3)

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Key Product Details

Source:	NS0
Accession #:	Q15582
Applications:	Bioactivity

View Full Technical Details for Recombinant Human beta IG-H3 Protein, CF

Technical Data
Citations (3) / Reviews

Product Specifications for Recombinant Human beta IG-H3 Protein, CF

Source	Mouse myeloma cell line, NS0-derived human beta IG-H3 protein Gly24-His683, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gly24
Predicted Molecular Mass	73 kDa
SDS-PAGE	73 kDa
Activity	Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. rh beta IG-H3, immobilized at 5 µg/mL (100 µL/well), can induce greater than 45% cell adhesion.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3409-BG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta IG-H3

Beta IG-H3, also known as TGFBI and RGD-CAP, is a matricellular adaptor protein that is induced in most cell types in response to TGF-beta stimulation (1-4). The human beta IG-H3 cDNA encodes a 683 amino acid (aa) precursor that includes a 23 aa signal sequence, one EMI domain, four FAS1 domains, and one RGD motif (1). Human beta IG-H3 shares 91% and 93% aa sequence identity with mouse and porcine beta IG-H3, respectively. beta IG-H3 is expressed as a 75 kDa protein with no post-translational additions (5). Following secretion, cleavages at multiple positions near the C-terminal end liberate peptides with pro-apoptotic activity (5,6). Peptides that encompass the RGD motif contribute to the pro-apoptotic effects of TGF-beta (6). FAS1 domains contain YH motifs that are characterized by conserved Tyr and His residues (7). The YH motifs in each of the FAS1 domains enable beta IG-H3 binding to matrix Fibronectin, Collagen I, and Collagen VI (3, 8 - 10) in addition to cell expressed Integrins alpha _V beta ₃, alpha _V beta ₅, and alpha ₃ beta ₁ (7, 8, 11, 12). The expression of beta IG-H3 is modulated at particular developmental stages in some cell types. It is upregulated in keratinocytes and immature dendritic cells but downregulated in osteoblasts (8, 11, 13). It promotes keratinocyte differentiation but blocks osteoblast differentiation (8,11). beta IG-H3 stimulates macrophage endocytosis and vascular endothelial cell proliferation and migration (12, 13). High glucose levels induce beta IG-H3 in renal proximal tubule cells which is predictive of diabetic nephropathy (3). Several point mutations (clustered in the fourth FAS1 domain) of beta IG-H3 are linked to different corneal dystrophies (14). beta IG-H3 is downregulated in many cancers (4, 15) and functions as a suppressor of tumorigenicity when overexpressed (2, 4, 15).

Item	Value
References	Skonier, J. et al. (1992) DNA Cell Biol. 11:511. Skonier, J. et al. (1994) DNA Cell Biol. 13:571. Lee, S-H. et al. (2003) Kidney Int. 64:1012. Zhao, Y.L. et al. (2002) Oncogene 21:7471. Andersen, R.B. et al. (2004) Biochemistry 43:16374. Kim, J-E. et al. (2003) Oncogene 22:2045. Kim, J-E. et al. (2002) J. Biol. Chem. 277:46159. Thapa, N. et al. (2005) Bone 36:232. Hanssen, E. et al. (2003) J. Biol. Chem. 278:24334. Billings, P.C. et al. (2002) J. Biol. Chem. 277:28003. Oh, J-E. et al. (2005) J. Biol. Chem. 280:21629. Nam, J-O. et al. (2003) J. Biol. Chem. 278:25902. Cao, W, et al. (2006) Blood 107:2777. Stewart, H.S. et al. (1999) Hum. Mutat. 14:126. Zhao, Y. et al. (2006) Mol. Carcinog. 45:84.
Long Name	TGF-beta Induced Gene H3
Entrez Gene IDs	7045 (Human); 21810 (Mouse)
Alternate Names	BIGH3EBMD, CDB1, CDG2, CDGG1transforming growth factor, beta-induced, 68kD, CSD, CSD1, CSD2, CSD3, Kerato-epithelin, LCD1, RGD-CAP, RGD-containing collagen-associated protein, TGFBI, TGFBIP, beta IG-H3, beta IGH3, transforming growth factor, beta-induced, 68kDa, transforming growth factor-beta-induced protein ig-h3

Citations for Recombinant Human beta IG-H3 Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human beta IG-H3 Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Yang et al. (2022-07-22), Transforming growth factor-beta induced protein regulates pulmonary fibrosis via the G-protein signaling modulator 2 /Snail axis Peptides, 2022-0155(0):170842.
PMID: 35872259
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-11-22), Migration of breast cancer cell lines in response to pulmonary laminin 332 Cancer Med, 2016-10(0).
PMID: 27878981
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Shelton L et al. (2009-04-22), Inhibition of human scleral fibroblast cell attachment to collagen type I by TGFBIp. Invest. Ophthalmol. Vis. Sci., 2009-050(8):3542-52.
PMID: 19387070

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