Source	Human embryonic kidney cell, HEK293-derived human B7-H7/HHLA2 protein Phe29-Asn344, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Phe29 & Ile30
Predicted Molecular Mass	37 kDa
SDS-PAGE	57-64 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human TMIGD2/CD28H (Catalog # 293-VE) is immobilized at 2.5 µg/mL (100 µL/ well), Recombinant Human B7‑H7/HHLA2 His-tag (Catalog # 10475-B7) that produces a 50% optimal binding response is found to be 0.8-4.8 µg/mL.

Recombinant Human B7-H7/HHLA2 His-tag Protein, CF Scientific Data Examples

	Recombinant Human B7-H7/HHLA2 His-tag Protein Binding Activity When Recombinant Human TMIGD2/CD28H ([[catalog Number:9236-TR]]) is immobilized at 2.5 μg/mL (100 μL/well), Recombinant Human B7 H7/HHLA2 His-tag ([[catalog Number:10475-B7]]) that produces a 50% optimal binding response is found to be 0.8-4.8 μg/mL.
	Recombinant Human B7-H7/HHLA2 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Human B7-H7/HHLA2 His-tag Protein (Catalog # 10475-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver stain, showing bands at 55-65 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10475-B7

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: B7-H7/HHLA2

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins (1, 2). Mature human B7-H7 consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5). B7‑H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). Soluble B7-H7 inhibits the proliferation of activated CD4⁺ and CD8⁺ T cells and their production of IFN-gamma, TNF-alpha, IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).

Item	Value
References	Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180. Mager, D.L. et al. (1999) Genomics 59:255. Flajnik, M.M. et al. (2012) Immunogenetics 64:571. Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.
Long Name	B7 Homolog 7
Entrez Gene IDs	11148 (Human); 102137704 (Cynomolgus Monkey)
Alternate Names	B7-H7, B7H7, HERV-H LTR-associating 2, HERV-H LTR-associating protein 2, HHLA2, Human endogenous retrovirus-H long terminal repeat-associating protein 2

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Recombinant Human B7-H7/HHLA2 His-tag Protein, CF

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