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Recombinant Human B7-H7/HHLA2 His-tag Protein, CF

Catalog # 10475-B7 | R&D Systems, Inc. a Bio-Techne Brand
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10475-B7-100

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human B7-H7/HHLA2 protein
Phe29-Asn344, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe29 & Ile30

Predicted Molecular Mass

37 kDa

SDS-PAGE

57-64 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human TMIGD2/CD28H (Catalog # 8316-TR) is immobilized at 2.5 µg/mL (100 µL/ well), Recombinant Human B7‑H7/HHLA2 His-tag (Catalog # 10475-B7) that produces a 50% optimal binding response is found to be 0.8-4.8 µg/mL.

Scientific Data Images for Recombinant Human B7-H7/HHLA2 His-tag Protein, CF

Recombinant Human B7-H7/HHLA2 His-tag Protein Binding Activity

Recombinant Human B7-H7/HHLA2 His-tag Protein Binding Activity

When Recombinant Human TMIGD2/CD28H (8316-TR) is immobilized at 2.5 μg/mL (100 μL/well), Recombinant Human B7 H7/HHLA2 His-tag (Catalog # 10475-B7) that produces a 50% optimal binding response is found to be 0.8-4.8 μg/mL.
Recombinant Human B7-H7/HHLA2 His-tag Protein SDS-PAGE

Recombinant Human B7-H7/HHLA2 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human B7-H7/HHLA2 His-tag Protein (Catalog # 10475-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver stain, showing bands at 55-65 kDa.

Formulation, Preparation and Storage

10475-B7
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.

Background: B7-H7/HHLA2

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins (1, 2). Mature human B7-H7 consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5). B7‑H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). Soluble B7-H7 inhibits the proliferation of activated CD4+ and CD8+ T cells and their production of IFN-gamma, TNF-alpha, IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).

References

  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Mager, D.L. et al. (1999) Genomics 59:255.
  4. Flajnik, M.M. et al. (2012) Immunogenetics 64:571.
  5. Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.

Long Name

B7 Homolog 7

Alternate Names

B7H7, HHLA2

Entrez Gene IDs

11148 (Human); 102137704 (Cynomolgus Monkey)

Gene Symbol

HHLA2

UniProt

Product Documents for Recombinant Human B7-H7/HHLA2 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human B7-H7/HHLA2 His-tag Protein, CF

For research use only

Reconstitution Calculator

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