Recombinant Human B7-H6 His-tag Protein, CF

Name: Recombinant Human B7-H6 His-tag Protein, CF
Brand: R&D Systems
SKU: 9309-B7
Rating: 5 (1 reviews)

Catalog # 9309-B7 | R&D Systems, Inc. a Bio-Techne Brand

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Product Datasheet / COA / SDS

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Key Product Details

Source:	HEK293
Accession #:	Q68D85
Applications:	Bioactivity

View Full Technical Details for Recombinant Human B7-H6 His-tag Protein, CF

Product Specifications for Recombinant Human B7-H6 His-tag Protein, CF

Source	Human embryonic kidney cell, HEK293-derived human B7-H6 protein Asp25-Ser262, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Asp25
Predicted Molecular Mass	28 kDa
SDS-PAGE	51-63 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H6 is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human NKp30/NCR3 Fc Chimera (Catalog # 1849-NK) that produces 50% of the optimal binding response is 0.02-0.1 μg/mL.

Scientific Data Examples for Recombinant Human B7-H6 His-tag Protein, CF

	Recombinant Human B7-H6 His-tag Protein Bioactivity When Recombinant Human B7-H6 (Catalog # 9309-B7) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human NKp30/NCR3 Fc Chimera (Catalog # 1849-NK) binds with an ED₅₀ of 0.02-0.1 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9309-B7

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: B7-H6

B7-H6 is a glycosylated member of the B7 family of immune co-stimulatory proteins (1 2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53%-56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7).

Item	Value
References	Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495. Li, Y. et al. (2011) J. Exp. Med. 208:703. Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. 108:6223. Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348. Byrd, A. et al. (2007) PLoS ONE 2:e1339.
Long Name	B7 Homolog 6
Entrez Gene IDs	374383 (Human); 102121659 (Cynomolgus Monkey)
Alternate Names	B7 homolog 6, B7-H6, B7H6, DKFZp686I21167, DKFZp686O24166, NCR3LG1

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Product Documents for Recombinant Human B7-H6 His-tag Protein, CF

Product Datasheets

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Recombinant Human B7-H6 His-tag Protein, CF

Catalog # 9309-B7 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human B7-H6 His-tag Protein, CF

Scientific Data Examples for Recombinant Human B7-H6 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: B7-H6

Citations for Recombinant Human B7-H6 His-tag Protein, CF

Customer Reviews for Recombinant Human B7-H6 His-tag Protein, CF (1)

Recombinant Human B7-H6 His-tag Protein, CF

Product Documents for Recombinant Human B7-H6 His-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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