Recombinant Human B4GAT1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6664-GT

R&D Systems, part of Bio-Techne
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6664-GT-050
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human B4GAT1 His-tag Protein (6664-GT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession #

O43505

Conjugate

Unconjugated

Applications

Enzyme Activity

View all beta-1,4-Glucuronyltransferase 1/B4GAT1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human beta-1,4-Glucuronyltransferase 1/B4GAT1 protein
His37-Cys415, with N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

43.8 kDa

SDS-PAGE

42-53 kDa under reducing conditions

Activity

Measured by its ability to transfer GlcA from UDP-GlcA to Xylose.
The specific actiivity is >55 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6664-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: beta-1,4-Glucuronyltransferase 1/B4GAT1

B4GAT1 was previously described in the literature as B3GNT1 (1). It is now characterized as a beta1,4 glucuronyltransferase that is responsible for the synthesis of a glucuronyl-beta 1,4-xylosyl disaccharide found on alpha-dystroglycan ( alpha- DG), a peripheral membrane protein that binds to several extracellular matrix components (2). Proper glycosylation of alpha-DG is critical to maintain structural integrity and force transmission between the cytoskeleton and the extracellular matrix for efficient signal transduction. Mutation of B4GAT1 will lead to failure of proper glycosylation of alpha-DG and loss of receptor binding of alpha-DG, therefore causes congenital muscular dystrophies (CMDs) (3). The enzymatic activity of recombinant human B4GAT1 was determined using a phosphatase-coupled assay (4) using xylose as acceptor substrate.

References

  1. Sasaki, K. et al. (1997) PNAS 94:14294.
  2. Willer, T. et al. (2014) eLife; 3: e03941.
  3. Barresi, R. and Campbell, K.P. (2006) J. Cell Sci. 119:199.
  4. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

beta-1,4-Glucuronyltransferase 1

Alternate Names

B3GNT1, iGAT, iGNT, MDDGA13

Entrez Gene IDs

11041 (Human); 108902 (Mouse); 293667 (Rat)

Gene Symbol

B4GAT1

UniProt

O43505 (Human)

Additional beta-1,4-Glucuronyltransferase 1/B4GAT1 Products

Product Documents for Recombinant Human B4GAT1 His-tag Protein, CF

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Product Specific Notices for Recombinant Human B4GAT1 His-tag Protein, CF

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