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Recombinant Human B4GalT7 His-tag Protein, CF

Catalog # 10663-GT | R&D Systems, Inc. a Bio-Techne Brand
Human Beta-1,4-Galactosyltransferase 7
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10663-GT-020

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Beta-1,4-Galactosyltransferase 7/B4GalT7 protein
Ser52-Ser327, with C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser52

Predicted Molecular Mass

32.5 kDa

SDS-PAGE

37-41 kDa, reducing conditions

Activity

Measured by its ability to transfer galactose from UDP-Galactose to D-Xylose.
The specific activity is >120 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human B4GalT7 His-tag Protein, CF

Recombinant Human B4GalT7 His-tag Protein Enzyme Activity Diagram

B4GalT7 attaches the first galactose in the common carbohydrate-protein (GlcA-beta -1,3-Gal-beta -1,3-Gal-beta -1,4-Xyl-beta1-O-Ser) linkage found in proteoglycans.

Recombinant Human B4GalT7 His-tag Protein SDS-PAGE.

1 μg/lane of Recombinant Human B4GalT7 His-tag (Catalog # 10663-GT) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 37-41 kDa.

Formulation, Preparation and Storage

10663-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Beta-1,4-Galactosyltransferase 7/B4GalT7

There are seven beta-1,4-galactosyltransferases that transfer galactose in a beta-1,4 linkage to acceptor sugars including GlcNAc, and Glc, and Xyl. B4GalT7, also known as galactosyltransferase I, catalyzes the addition of the first galactose to the glycosaminoglycan-protein tetrasaccharide linkage (GlcA-beta -1,3-Gal-beta -1,3-Gal-beta -1,4-Xyl-beta1-O-Ser) in proteoglycans (1). Proteoglycans are structural components of the extracellular matrix that is found between cells in connective tissues (2). B4GalT7 resides in cis-Golgi apparatus and is a type II membrane protein and differs from the other six B4GalTs for lacking a conserved Cys residue (3). Mutations in the gene of B4GalT7 have been associated with Ehlers-Danlos syndrome (4, 5) and Larsen syndrome (6), likely due to reduced glycosaminoglycan synthesis in decorin and biglycan, which further results in delayed wound repair, altered migration, adhesion and contractility of patient fibroblasts (7, 8). The activity of this enzyme has been measured with a phosphatase-coupled method (9).

References

  1. Almeida, R. et al. (1999) J. Biol. Chem. 274: 26165.
  2. Iozzo, RV and Schaefer, L (2015) Matrix Biology 42:11.
  3. Tsutsui, Y., Ramakrishnan, B. and Qasba, P.K. (2013) J. Biol. Chem. 288:31963.
  4. Furukawa, K, and Okajima, T (2002) Biochim. Biophys. Acta 1573:377.
  5. Okajima, T. et al. (1999) J. Biol. Chem. 274:28841.
  6. Cartault, F. et al. (2015) Eur. J. Hum. Genet. 23:49.
  7. Seidler, D.G. et al. (2006). J. Mol. Med. 84:583.
  8. Götte, M. et al. (2008). Hum. Mol. Genet. 17:996.
  9. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

UDP-Gal:Beta-GlcNAc Beta-1,4-Galactosyltransferase 7

Alternate Names

EDSP1, EDSSLA, EDSSPD1, XGALT1, XGPT, XGPT1

Entrez Gene IDs

11285 (Human); 218271 (Mouse)

Gene Symbol

B4GALT7

UniProt

Additional Beta-1,4-Galactosyltransferase 7/B4GalT7 Products

Product Documents for Recombinant Human B4GalT7 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human B4GalT7 His-tag Protein, CF

For research use only

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