Product Specifications for Recombinant Human B4GalT7 His-tag Protein, CF
Human embryonic kidney cell, HEK293-derived human Beta-1,4-Galactosyltransferase 7/B4GalT7 protein Ser52-Ser327, with C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
37-41 kDa, reducing conditions
Measured by its ability to transfer galactose from UDP-Galactose to D-Xylose. The specific activity is >120 pmol/min/μg, as measured under the described conditions.
Scientific Data Examples for Recombinant Human B4GalT7 His-tag Protein, CF
Recombinant Human B4GalT7 His-tag Protein Enzyme Activity Diagram
B4GalT7 attaches the first galactose in the common carbohydrate-protein (GlcA-beta -1,3-Gal-beta -1,3-Gal-beta -1,4-Xyl-beta1-O-Ser) linkage found in proteoglycans.
Recombinant Human B4GalT7 His-tag Protein SDS-PAGE.
1 μg/lane of Recombinant Human B4GalT7 His-tag (Catalog # 10663-GT) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 37-41 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
There are seven beta-1,4-galactosyltransferases that transfer galactose in a beta-1,4 linkage to acceptor sugars including GlcNAc, and Glc, and Xyl. B4GalT7, also known as galactosyltransferase I, catalyzes the addition of the first galactose to the glycosaminoglycan-protein tetrasaccharide linkage (GlcA-beta -1,3-Gal-beta -1,3-Gal-beta -1,4-Xyl-beta1-O-Ser) in proteoglycans (1). Proteoglycans are structural components of the extracellular matrix that is found between cells in connective tissues (2). B4GalT7 resides in cis-Golgi apparatus and is a type II membrane protein and differs from the other six B4GalTs for lacking a conserved Cys residue (3). Mutations in the gene of B4GalT7 have been associated with Ehlers-Danlos syndrome (4, 5) and Larsen syndrome (6), likely due to reduced glycosaminoglycan synthesis in decorin and biglycan, which further results in delayed wound repair, altered migration, adhesion and contractility of patient fibroblasts (7, 8). The activity of this enzyme has been measured with a phosphatase-coupled method (9).
Almeida, R. et al. (1999) J. Biol. Chem. 274: 26165.
Iozzo, RV and Schaefer, L (2015) Matrix Biology 42:11.
Tsutsui, Y., Ramakrishnan, B. and Qasba, P.K. (2013) J. Biol. Chem. 288:31963.
Furukawa, K, and Okajima, T (2002) Biochim. Biophys. Acta 1573:377.
Okajima, T. et al. (1999) J. Biol. Chem. 274:28841.
Cartault, F. et al. (2015) Eur. J. Hum. Genet. 23:49.
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