Recombinant Human B3GAT1 Protein, CF

Name: Recombinant Human B3GAT1 Protein, CF
Brand: R&D Systems
SKU: 8560-GT

Catalog # 8560-GT | R&D Systems, Inc. a Bio-Techne Brand

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Key Product Details

Source:	CHO
Accession #:	Q9P2W7
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human B3GAT1 Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human B3GAT1 Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived human beta-1,3-Glucuronyltransferase 1/B3GAT1 protein His25-Ile334, with N-terminal 6-His tag
Purity	>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	His
Predicted Molecular Mass	36 kDa
SDS-PAGE	36 kDa
Activity	Measured by its ability to transfer GlcA from UDP-GlcA to lactose. The specific activity is >750 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8560-GT

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: beta-1,3-Glucuronyltransferase 1/B3GAT1

B3GAT1 is a key enzyme involved in human natural killer1 (HNK1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta 14GlcNAc) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO₃-3GlcA beta 1-3Gal beta 1-4GlcNAc (1, 2). The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol (3). The HNK1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell-cell and cell-substratum interaction and recognition during the development of the nervous system (4). Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N-terminal cytoplasmic domain and a single pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method (5).

Item	Value
References	Terayama, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6093. Shogo, O. et al. (1992) J. Biol. Chem. 267: 22711. Kakuda, S. et al. (2005) Glycobiology 2:203. Bollensen, E. and Schachner, M. (1987) Neurosci Lett. 82:77. Wu, Z.L. et al. (2011) Glycobiology 21:727.
Long Name	UDP-GlcUA:glycoprotein beta-1,3-Glucuronyltransferase
Entrez Gene IDs	27087 (Human); 76898 (Mouse); 117108 (Rat)
Alternate Names	B3GAT1, Beta-1,3-glucuronyltransferase 1, CD57, EC 2.4.1.135, GLCATP, GLCATPGLCUATP, GLCUATP, GlcAT-P, Glucuronosyltransferase P, HNK1, LEU7, NK-1, NK1, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P), galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, glcUAT-P

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Recombinant Human B3GAT1 Protein, CF

Catalog # 8560-GT | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human B3GAT1 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: beta-1,3-Glucuronyltransferase 1/B3GAT1

Citations for Recombinant Human B3GAT1 Protein, CF

Product Documents for Recombinant Human B3GAT1 Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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