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Recombinant Human B3GalT5 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10555-GT

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10555-GT-020

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human B3GalT5 protein
Asn29-Val310, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn29

Predicted Molecular Mass

34.7 kDa

SDS-PAGE

40-45 kDa, under reducing conditions.

Activity

Measured by its ability to transfer galactose from UDP-galactose to N-Acetyl-alpha -D-glucosamine.
The specific activity is >200 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human B3GalT5 His-tag Protein, CF

Recombinant Human B3GalT5 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human B3GalT5 His-tag Protein (Catalog # 10555-GT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-45 kDa.

Formulation, Preparation and Storage

10555-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: B3GalT5

B3GalT5 is a glycosyltransferase in the beta-1,3-galactosyltransferase (B3GalT) family that contains 5 members. It is synthesized as type II membrane-bound glycoproteins in the Golgi apparatus (1). The enzyme catalyzes the synthesis of type 1 lactosamine structure (Gal beta1-3GlcNAc) in type 1 Lewis antigens (Lea and Leb). Lea and Leb are elevated in gastrointestinal and pancreatic cancers (2). Sialylated Lea (Neu5Ac alpha2-3Gal beta1-3[Fuc alpha1-4]GlcNAc beta), also known as CA19-9, is a tumor marker in the gastrointestinal tract (Stomach, colon, and pancrease) (3). Down-regulation of B3GalT5 will reduce the level of CA19-9 (4, 5). In contrast, beta-1,4-galactosyltransferases synthesize type 2 lactosamine structure (Gal beta1-4GlcNAc) that is found in type 2 Lewis antigens (Lex and Ley). B3GalT5 is also known as SSEA-3 synthase and synthesizes the structure Gal beta1-3GalNAc in stage-specific embryonic antigen-3 (SSEA-3) antigen (6). SSEA-3 plays a key role in identifying many types of mammalian cells with pluripotent and stem cell-like characteristics (7). It also catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the Core-3 O-linked glycan GlcNAc beta1-3GalNAc structure (8). The activity of this enzyme has been measured with a phosphatase-coupled method (9).

References

  1. Isshiki S. et al. (1999) J. Biol. Chem. 274:1249.
  2. Holgersson J and Löfling J. (2006) Glycobiology 16:584.
  3. Magnani, JL (2004). Arch. Biochem. Biophys. 426:122.
  4. Mare L. and Trinchera M. (2004) Eur. J. Biochem. 271:186.
  5. Zulueta, A.et al. (2014) FASEB J. 28:946.
  6. Zhou D. et al. (2000) J. Biol. Chem. 275:22631.
  7. Kuroda, Y. et al. (2010) Proc. Natl. Acad. Sci. U.S.A. 107: 8639.
  8. Zhou D. et al. (1999) Eur. J. Biochem. 263:571..
  9. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5

Alternate Names

B3Gal-T5, B3GalT-V, B3GalTx, B3T5, beta-1,3-Galactosyltransferase 5, beta3Gal-T5, GlcNAc-Beta-1,3-Galactosyltransferase 5, GLCT5, UDP-Gal:Beta-GlcNAc Beta-1,3-Galactosyltransferase

Entrez Gene IDs

10317 (Human); 93961 (Mouse); 288161 (Rat)

Gene Symbol

B3GALT5

UniProt

Additional B3GalT5 Products

Product Documents for Recombinant Human B3GalT5 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human B3GalT5 His-tag Protein, CF

For research use only

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