Recombinant Human B3GalT5 His-tag Protein, CF

Name: Recombinant Human B3GalT5 His-tag Protein, CF
Brand: R&D Systems
SKU: 10555-GT
Price: 460 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10555-GT

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Learn more about Fluorescent Glycan Labeling and Detection

Source:	CHO
Accession #:	Q9Y2C3.1
Applications:	Enzyme Activity

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Product Specifications for Recombinant Human B3GalT5 His-tag Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived human B3GalT5 protein Asn29-Val310, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Asn29
Predicted Molecular Mass	34.7 kDa
SDS-PAGE	40-45 kDa, under reducing conditions.
Activity	Measured by its ability to transfer galactose from UDP-galactose to N-Acetyl-alpha -D-glucosamine. The specific activity is >200 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human B3GalT5 His-tag Protein, CF

	Recombinant Human B3GalT5 His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human B3GalT5 His-tag Protein (Catalog # 10555-GT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-45 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10555-GT

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: B3GalT5

B3GalT5 is a glycosyltransferase in the beta-1,3-galactosyltransferase (B3GalT) family that contains 5 members. It is synthesized as type II membrane-bound glycoproteins in the Golgi apparatus (1). The enzyme catalyzes the synthesis of type 1 lactosamine structure (Gal beta 1-3GlcNAc) in type 1 Lewis antigens (Le^a and Le^b). Le^a and Le^b are elevated in gastrointestinal and pancreatic cancers (2). Sialylated Le^a (Neu5Ac alpha 2-3Gal beta 1-3[Fuc alpha 1-4]GlcNAc beta ), also known as CA19-9, is a tumor marker in the gastrointestinal tract (Stomach, colon, and pancrease) (3). Down-regulation of B3GalT5 will reduce the level of CA19-9 (4, 5). In contrast, beta-1,4-galactosyltransferases synthesize type 2 lactosamine structure (Gal beta 1-4GlcNAc) that is found in type 2 Lewis antigens (Le^x and Le^y). B3GalT5 is also known as SSEA-3 synthase and synthesizes the structure Gal beta 1-3GalNAc in stage-specific embryonic antigen-3 (SSEA-3) antigen (6). SSEA-3 plays a key role in identifying many types of mammalian cells with pluripotent and stem cell-like characteristics (7). It also catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the Core-3 O-linked glycan GlcNAc beta 1-3GalNAc structure (8). The activity of this enzyme has been measured with a phosphatase-coupled method (9).

Item	Value
References	Isshiki S. et al. (1999) J. Biol. Chem. 274:1249. Holgersson J and Löfling J. (2006) Glycobiology 16:584. Magnani, JL (2004). Arch. Biochem. Biophys. 426:122. Mare L. and Trinchera M. (2004) Eur. J. Biochem. 271:186. Zulueta, A.et al. (2014) FASEB J. 28:946. Zhou D. et al. (2000) J. Biol. Chem. 275:22631. Kuroda, Y. et al. (2010) Proc. Natl. Acad. Sci. U.S.A. 107: 8639. Zhou D. et al. (1999) Eur. J. Biochem. 263:571.. Wu, Z.L. et al. (2011) Glycobiology 21:727.
Long Name	UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5
Entrez Gene IDs	10317 (Human); 93961 (Mouse); 288161 (Rat)
Alternate Names	B3Gal-T5, B3GalT-V, B3GalT5, B3GalTx, B3T5, Beta-1,3-GalTase 5, Beta3GalT5, EC 2.4.1, EC 2.4.1.-, GLCT5, GlcNAc-Beta-1,3-Galactosyltransferase 5, UDP-Gal:Beta-GlcNAc Beta-1,3-Galactosyltransferase, UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 5, UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5,10Beta-3-Gx-T5, UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5, beta-1,3-Galactosyltransferase 5, beta3Gal-T5

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Recombinant Human B3GalT5 His-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human B3GalT5 His-tag Protein, CF

Scientific Data Examples for Recombinant Human B3GalT5 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: B3GalT5

Citations for Recombinant Human B3GalT5 His-tag Protein, CF

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