Recombinant Human Apolipoprotein E3/ApoE3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11781-AE

R&D Systems, part of Bio-Techne
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11781-AE-100
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Key Product Details

Source

HEK293

Accession #

P02649.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Apolipoprotein E3/ApoE3 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Apolipoprotein E3/ApoE3 protein
Lys19-His318

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys19

Predicted Molecular Mass

34 kDa

SDS-PAGE

34-40 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human Apolipoprotein E3/ApoE3 binds to Recombinant Human VLDLR Protein  (Catalog # 8444-VL) with an ED50 of 0.0250‑0.300 μg/mL.

Scientific Data Images for Recombinant Human Apolipoprotein E3/ApoE3 Protein, CF

Recombinant Human Apolipoprotein E3/ApoE3 Protein Bioactivity.

Measured by its binding ability in a functional ELISA. Recombinant Human Apolipoprotein E3/ApoE3 Protein binds to Recombinant Human VLDLR Protein (8444-VL) with an ED50 of 0.0250-0.300 μg/mL.

Recombinant Human Apolipoprotein E3/ApoE3 Protein SDS-PAGE.

2 μg/lane of Recombinant Human Apolipoprotein E3/ApoE3 Protein (Catalog # 11781-AE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-40 kDa, under reducing conditions.

Formulation, Preparation and Storage

11781-AE
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and TCEP with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein E3/ApoE3

Apolipoprotein E (ApoE) is a polymorphic lipid-transport protein with three major human isoforms—ApoE2, ApoE3, and ApoE4—differing by single amino acid substitutions at residues 112 and 158, which significantly alter their structure and function [1, 2].  All isoforms share a two-domain structure: the N-terminal domain mediates receptor binding, while the C-terminal domain facilitates lipid binding and oligomerization [2]. ApoE3 (Cys112/Arg158) is the most common and functionally neutral isoform, while ApoE2 (Cys112/Cys158) has reduced affinity for LDL receptors, often leading to type III hyperlipoproteinemia in homozygotes [1, 4]. ApoE4 (Arg112/Arg158) exhibits a more compact and less stable structure due to domain interaction, predisposing it to pathological effects in the brain [2, 5]. Functionally, ApoE isoforms differentially regulate neuronal metabolism: ApoE2 enhances glycolytic activity and hexokinase expression, promoting neuroprotection, whereas ApoE4 impairs these pathways, increasing vulnerability to aging and Alzheimer's disease (AD) [1]. Recent transcriptomic and epigenomic profiling of human microglia in an AD xenotransplantation model revealed that ApoE isoforms distinctly shape gene expression and chromatin accessibility. ApoE4 drives pro-inflammatory and neurodegenerative signatures, while ApoE2 supports homeostatic microglial states [3]. Clinically, ApoE genotyping is widely used to assess AD risk, with ApoE4 being the strongest genetic risk factor and ApoE2 offering relative protection [4, 5]. Therapeutic strategies targeting ApoE include isoform-specific modulation and gene editing. Additionally, ApoE isoforms are valuable tools in translational research for drug screening, biomarker discovery, and personalized medicine [4].

References

  1. Zhang X, et al.  (2023)  Cells 12:410.
  2. Horn JVC, et al. (2023)  Mol Cell Biochem 478: 173.
  3. Murphy KB, et al. (2025). Nat Commun 16:4883.
  4. Mahley RW, et al. (2012) J Lipid Res 53: 539.
  5. Liu CC, et al.  (2013) Nat Rev Neurol  9: 106.

Alternate Names

AD2

Entrez Gene IDs

348 (Human); 11816 (Mouse)

Gene Symbol

APOE

UniProt

P02649.1

Additional Apolipoprotein E3/ApoE3 Products

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Product Specific Notices for Recombinant Human Apolipoprotein E3/ApoE3 Protein, CF

For research use only

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