Recombinant Human APCDD1 His-tag Protein, CF

Name: Recombinant Human APCDD1 His-tag Protein, CF
Brand: rnd
SKU: 10141-AP

Catalog # 10141-AP

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Key Product Details

Source:	NS0
Accession #:	Q8J025
Applications:	Bioactivity

View Full Technical Details for Recombinant Human APCDD1 His-tag Protein, CF

Product Specifications

Source	Mouse myeloma cell line, NS0-derived human APCDD1 protein Leu27-His492, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser25 & Leu27
Predicted Molecular Mass	54 kDa
SDS-PAGE	50-70 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When 300 ng/mL of Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324) is immobilized onto Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human APCDD His-tag (Catalog # 10141-AP) with an ED₅₀ of 0.6-4.8 μg/mL.

Recombinant Human APCDD1 His-tag Protein, CF Scientific Data Examples

	Recombinant Human APCDD1 His-tag Protein Binding Activity When 300 ng/mL of Biontinylated Recombinant Mouse Wnt-3a (Catalog # BT1324) is immobilized onto Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human APCDD1 (Catalog # 10141-AP) with an ED₅₀ of 0.6-4.8 μg/mL.
	Recombinant Human APCDD1 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Mouse APCDD1 His-tag (Catalog # 10141-AP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10141-AP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: APCDD1

APCDD1 (Adenomatosis Polyposis Coli Down-Regulated 1) is a membrane bound glycoprotein that is an endogenous inhibitor of the Wnt signaling pathway (1, 2). Inhibition of Wnt signaling by APCDD1 plays a role in adipocyte differentiation as well as pathogenesis of disease (2-4). Mature human APCDD1 consists of a 465 amino acid (aa) extracellular domain (ECD), a 19 aa transmembrane domain, and a 1 aa cytoplasmic region. Human APCDD1 shares a 95% and 94% amino acid sequence similarity with rat and mouse respectively. APCDD1 interacts in vitro with Wnt-3a and LRP5 (5). It is expressed in a broad repertoire of cell types which might regulate a diversity of biological processes controlled by Wnt signaling, including breast cancer cell invasion (6), osteogenic differentiation of human dental follicle cells (7), vascular remodeling and barrier maturation of retinal blood vessels (4) and hair follicle miniaturization (5). Extracellular domain of APCDD1 has been shown to co-immunoprecipitate with recombinant Wnt-3a and LRP5 (1), suggesting that APCDD1 can modulate the Wnt pathway by potential interactions with Wnt-3a and LRP5 at the cell surface.

Item	Value
References	Mazzoni, J. et al. (2017) Neuron. 96:1055. Yiew, N.K.H. et al. (2017) J. Biol. Chem. 292:6312. Shimomura, Y. (2010) Nature 464:1043. Kandimalla, R. (2017) Oncogenesis 6:e308. Yutaka Shimomura, et al. (2010) Nature 464:1043. Sung-Gook CHO, (2017) Oncology Letters 14:4845. Viale-Bouroncle S. et al. (2015) Biochem Biophys Res Commun 457(3):314.
Long Name	APC Down Regulated 1
Entrez Gene IDs	147495 (Human); 494504 (Mouse); 689616 (Rat)
Alternate Names	APC Down Regulated 1, APCDD1, Adenomatosis polyposis coli down-regulated 1 protein, B7323, DRAPC1, HYPT1, adenomatosis polyposis coli down-regulated 1, protein APCDD1

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