Mouse myeloma cell line, NS0-derived human APCDD1 protein Leu27-His492, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Ser25 & Leu27
Predicted Molecular Mass
50-70 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When 300 ng/mL of Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324) is immobilized onto Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human APCDD His-tag (Catalog # 10141-AP) with an ED50 of 0.6-4.8 μg/mL.
Recombinant Human APCDD1 His-tag Protein, CF Scientific Data Examples
Recombinant Human APCDD1 His-tag Protein Binding Activity
When 300 ng/mL of Biontinylated Recombinant Mouse Wnt-3a (Catalog # BT1324) is immobilized onto Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human APCDD1 (Catalog # 10141-AP) with an ED50 of 0.6-4.8 μg/mL.
Recombinant Human APCDD1 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Mouse APCDD1 His-tag (Catalog # 10141-AP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 1 mg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
APCDD1 (Adenomatosis Polyposis Coli Down-Regulated 1) is a membrane bound glycoprotein that is an endogenous inhibitor of the Wnt signaling pathway (1, 2). Inhibition of Wnt signaling by APCDD1 plays a role in adipocyte differentiation as well as pathogenesis of disease (2-4). Mature human APCDD1 consists of a 465 amino acid (aa) extracellular domain (ECD), a 19 aa transmembrane domain, and a 1 aa cytoplasmic region. Human APCDD1 shares a 95% and 94% amino acid sequence similarity with rat and mouse respectively. APCDD1 interacts in vitro with Wnt-3a and LRP5 (5). It is expressed in a broad repertoire of cell types which might regulate a diversity of biological processes controlled by Wnt signaling, including breast cancer cell invasion (6), osteogenic differentiation of human dental follicle cells (7), vascular remodeling and barrier maturation of retinal blood vessels (4) and hair follicle miniaturization (5). Extracellular domain of APCDD1 has been shown to co-immunoprecipitate with recombinant Wnt-3a and LRP5 (1), suggesting that APCDD1 can modulate the Wnt pathway by potential interactions with Wnt-3a and LRP5 at the cell surface.
Mazzoni, J. et al. (2017) Neuron. 96:1055.
Yiew, N.K.H. et al. (2017) J. Biol. Chem. 292:6312.
Shimomura, Y. (2010) Nature 464:1043.
Kandimalla, R. (2017) Oncogenesis 6:e308.
Yutaka Shimomura, et al. (2010) Nature 464:1043.
Sung-Gook CHO, (2017) Oncology Letters 14:4845.
Viale-Bouroncle S. et al. (2015) Biochem Biophys Res Commun 457(3):314.
Which Brands are Currently Available on bio-techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments and Simple Plex Assays are available to quote. ProteinSimple branded instrument consumables and ACD branded products will be available on bio-techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
Setting the standard in quality research reagents for over 30 years
A trusted leader in quality life science reagents
Your trusted supplier for innovative and high performance life science reagents
Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis
Proprietary RNAscope® technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity
A world leader in developing liquid biopsy based diagnostics