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Recombinant Human APCDD1 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10141-AP

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10141-AP-100

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human APCDD1 protein
Leu27-His492, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser25 & Leu27

Predicted Molecular Mass

54 kDa

SDS-PAGE

50-70 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When 300 ng/mL of Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324) is immobilized onto Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human APCDD His-tag (Catalog # 10141-AP) with an ED50 of 0.6-4.8 μg/mL.

Scientific Data Images for Recombinant Human APCDD1 His-tag Protein, CF

Recombinant Human APCDD1 His-tag Protein Binding Activity

Recombinant Human APCDD1 His-tag Protein Binding Activity

When 300 ng/mL of Biontinylated Recombinant Mouse Wnt-3a (Catalog # BT1324) is immobilized onto Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human APCDD1 (Catalog # 10141-AP) with an ED50 of 0.6-4.8 μg/mL.
Recombinant Human APCDD1 His-tag Protein SDS-PAGE

Recombinant Human APCDD1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse APCDD1 His-tag (Catalog # 10141-AP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.

Formulation, Preparation and Storage

10141-AP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APCDD1

APCDD1 (Adenomatosis Polyposis Coli Down-Regulated 1) is a membrane bound glycoprotein that is an endogenous inhibitor of the Wnt signaling pathway (1, 2). Inhibition of Wnt signaling by APCDD1 plays a role in adipocyte differentiation as well as pathogenesis of disease (2-4). Mature human APCDD1 consists of a 465 amino acid (aa) extracellular domain (ECD), a 19 aa transmembrane domain, and a 1 aa cytoplasmic region. Human APCDD1 shares a 95% and 94% amino acid sequence similarity with rat and mouse respectively. APCDD1 interacts in vitro with Wnt-3a and LRP5 (5). It is expressed in a broad repertoire of cell types which might regulate a diversity of biological processes controlled by Wnt signaling, including breast cancer cell invasion (6), osteogenic differentiation of human dental follicle cells (7), vascular remodeling and barrier maturation of retinal blood vessels (4) and hair follicle miniaturization (5). Extracellular domain of APCDD1 has been shown to co-immunoprecipitate with recombinant Wnt-3a and LRP5 (1), suggesting that APCDD1 can modulate the Wnt pathway by potential interactions with Wnt-3a and LRP5 at the cell surface.

References

  1. Mazzoni, J. et al. (2017) Neuron. 96:1055.
  2. Yiew, N.K.H. et al. (2017) J. Biol. Chem. 292:6312.
  3. Shimomura, Y. (2010) Nature 464:1043.
  4. Kandimalla, R. (2017) Oncogenesis 6:e308.
  5. Yutaka Shimomura, et al. (2010) Nature 464:1043.
  6. Sung-Gook CHO, (2017) Oncology Letters 14:4845.
  7. Viale-Bouroncle S. et al. (2015) Biochem Biophys Res Commun 457(3):314.

Long Name

APC Down Regulated 1

Alternate Names

APC Down Regulated 1, DRAPC1, HYPT1

Entrez Gene IDs

147495 (Human); 494504 (Mouse); 689616 (Rat)

Gene Symbol

APCDD1

UniProt

Additional APCDD1 Products

Product Documents for Recombinant Human APCDD1 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human APCDD1 His-tag Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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